IKK alpha Antibody (14A231) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27409B

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP2-27409AF350

Alexa Fluor 405

NBP2-27409AF405

Alexa Fluor 488

NBP2-27409AF488

Alexa Fluor 532

NBP2-27409AF532

Alexa Fluor 594

NBP2-27409AF594

Alexa Fluor 647

NBP2-27409AF647

Alexa Fluor 700

NBP2-27409AF700

Alexa Fluor 750

NBP2-27409AF750

Allophycocyanin

NBP2-27409APC

DyLight 350

NBP2-27409UV

DyLight 405

NBP2-27409V

DyLight 488

NBP2-27409G

DyLight 550

NBP2-27409R

DyLight 594

NB100-56704DL594

DyLight 650

NBP2-27409C

DyLight 680

NBP2-27409FR

DyLight 755

NBP2-27409IR

FITC

NBP2-27409F

HRP

NBP2-27409H

Janelia Fluor 549

NBP2-27409JF549

Janelia Fluor 646

NBP2-27409JF646

mFluor Violet 450 SE

NBP2-27409MFV450

mFluor Violet 500 SE

NBP2-27409MFV500

mFluor Violet 610 SE

NBP2-27409MFV610

PE

NBP2-27409PE

PerCP

NBP2-27409PCP

Forumulation

Catalog #

BSA Free

NB100-56704

Azide Free

NBP2-27409

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Applications

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 14A231

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IKK alpha Antibodies »

Product Summary for IKK alpha Antibody (14A231) [Biotin]

Immunogen

This antibody was raised against a His-tagged full-length human IKK alpha protein.

Reactivity Notes

New World Monkey

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Applications for IKK alpha Antibody (14A231) [Biotin]

Application

Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IKK alpha

IKK-alpha (I-Kappa-B kinase-alpha) is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. Activation of NFKB by TNF requires phosphorylation of IKK-alpha at threonine 23 and serine 176 by AKT1 and NIK respectively (3). It has been demonstrated that IKK-alpha can accumulate in the nucleus after cytokine exposure (4). In the nucleus IKK-alpha interacts with CREB-binding protein in conjunction with RELA to be recruited to the NF-kappa-B-responsive promoters in order to mediate the cytokine-induced phosphorylation and subsequent acetylation of specific residues in histone H3 (5).

Long Name

IkB Kinase alpha

Alternate Names

CHUK, IkBKalpha, IKK1, NFKBIKA, TCF16

Gene Symbol

CHUK

Additional IKK alpha Products

Product Documents for IKK alpha Antibody (14A231) [Biotin]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for IKK alpha Antibody (14A231) [Biotin]

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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