IKK alpha Antibody (2M10E3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15862

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15862-100ul
NBP3-15862-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2M10E3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IKK alpha Antibodies »

Product Summary for IKK alpha Antibody (2M10E3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IKK alpha (NP_001269.3). MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIKSCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNILIHDVPLLAMEYCSG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IKK alpha Antibody (2M10E3)

Western Blot: IKK alpha Antibody (2M10E3) [NBP3-15862]

Western Blot: IKK alpha Antibody (2M10E3) [NBP3-15862]

Western Blot: IKK alpha Antibody (2M10E3) [NBP3-15862] - Western blot analysis of extracts of various cell lines, using IKK alpha antibody (NBP3-15862) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Applications for IKK alpha Antibody (2M10E3)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IKK alpha

IKK-alpha (I-Kappa-B kinase-alpha) is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. Activation of NFKB by TNF requires phosphorylation of IKK-alpha at threonine 23 and serine 176 by AKT1 and NIK respectively (3). It has been demonstrated that IKK-alpha can accumulate in the nucleus after cytokine exposure (4). In the nucleus IKK-alpha interacts with CREB-binding protein in conjunction with RELA to be recruited to the NF-kappa-B-responsive promoters in order to mediate the cytokine-induced phosphorylation and subsequent acetylation of specific residues in histone H3 (5).

Long Name

IkB Kinase alpha

Alternate Names

CHUK, IkBKalpha, IKK1, NFKBIKA, TCF16

Gene Symbol

CHUK

Additional IKK alpha Products

Product Documents for IKK alpha Antibody (2M10E3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IKK alpha Antibody (2M10E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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