IKK alpha Antibody (4D11)

Novus Biologicals, part of Bio-Techne | Catalog # H00001147-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4D11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IKK alpha Antibodies »

Product Specifications

Immunogen

CHUK (NP_001269, 646 a.a. ~ 745 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQTSAWLPPTSAEHDHSLSCVVTPQDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDWSWLTE

Localization

Cytoplasmic and Nuclear

Specificity

CHUK - conserved helix-loop-helix ubiquitous kinase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for IKK alpha Antibody (4D11)

Immunocytochemistry/ Immunofluorescence: IKK alpha Antibody (4D11) [H00001147-M01]

Immunocytochemistry/ Immunofluorescence: IKK alpha Antibody (4D11) [H00001147-M01]

Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (4D11) [H00001147-M01] - Analysis of monoclonal antibody to CHUK on HeLa cell . Antibody concentration 10 ug/ml.
ELISA: IKK alpha Antibody (4D11) [H00001147-M01]

ELISA: IKK alpha Antibody (4D11) [H00001147-M01]

ELISA: IKK alpha Antibody (4D11) [H00001147-M01] - Detection limit for recombinant GST tagged CHUK is 0.03 ng/ml as a capture antibody.

Applications for IKK alpha Antibody (4D11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IKK alpha

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

Long Name

IkB Kinase alpha

Alternate Names

CHUK, IkBKalpha, IKK1, NFKBIKA, TCF16

Entrez Gene IDs

1147 (Human)

Gene Symbol

CHUK

OMIM

600664 (Human)

UniProt

NP_001269 (Human)

Additional IKK alpha Products

Product Documents for IKK alpha Antibody (4D11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IKK alpha Antibody (4D11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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