Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IKK epsilon Antibodies »

Product Specifications

Immunogen

IKK epsilon/IKBKE phospho peptide corresponding to a region of the human protein surrounding pT501 conjugated to KLH. (Uniprot: Q14164)

Reactivity Notes

This phospho specific polyclonal antibody is specific for phosphorylated pT501 human IKK epsilon/IKBKE. Reactivity with non-phosphorylated IKK epsilon/IKBKE is minimal. Cross reactivity with pT501 phosphorylated IKK epsilon/IKBKE from mouse, rat or other species has not been determined.

Modification

p Thr501

Specificity

This phospho specific polyclonal antibody is specific for phosphorylated pT501 human IKK epsilon/IKBKE. Reactivity with non-phosphorylated IKK epsilon/IKBKE is minimal. Cross reactivity with pT501 phosphorylated IKK epsilon/IKBKE from mouse, rat or other species has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody was affinity purified from monospecific antiserum by immunoaffinity purification against the phosphopeptide and cross adsorption against the non-phosphorylated form of the peptide followed by non-adsorption against a non-specific peptide backbone to further reduce non-specific reactivity

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Applications for IKK epsilon/IKBKE [p Thr501] Antibody

Application
Recommended Usage

ELISA

1:5000-1:25000

Western Blot

1:500-1:3000
Application Notes
This product is tested in ELISA, western blotting, and although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. An 85 kDa band corresponding to human IKKe is detected. HeLa cells or TNF inducible KBM-5 cells can be used as a positive control. Researchers should determine optimal titers for other applications.

See Simple Western Antibody Database for Simple Western validation: tested in HeLa-/+EGF, Jurkat/TNF; antibody dilution of 1:50; separated by charge

Reviewed Applications

Read 1 review rated 5 using NBP1-77829 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: IKK epsilon

Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-kB activity. IkB proteins are phosphorylated by an IkB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg. Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKe is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFaor IL-1. LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa.

Long Name

IkB Kinase epsilon

Alternate Names

IKBKE, IKK-I

Entrez Gene IDs

9641 (Human)

Gene Symbol

IKBKE

UniProt

Q14164

Additional IKK epsilon Products

Product Documents for IKK epsilon/IKBKE [p Thr501] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IKK epsilon/IKBKE [p Thr501] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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