IKK gamma Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-215

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all IKK gamma Antibodies »

Product Specifications

Immunogen

N-terminus of the human IKK-gamma protein (proprietary).

Reactivity Notes

Human. Other species have not been tested.

Specificity

Reacts with the residues 2-13 [NRHLWKSQLCEM] of the IKK-g protein. Recognizes both human isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IKK gamma Antibody - BSA Free

Western Blot: IKK gamma AntibodyBSA Free [NB600-215]

Western Blot: IKK gamma AntibodyBSA Free [NB600-215]

Western Blot: IKK-gamma Antibody [NB600-215] - WB detection of IKK-gamma in Interleuking 1 stimulated 293 cells at 1:500 dilution.

Applications for IKK gamma Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:100-1:2000
Application Notes
Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IKK gamma

Pro-inflammatory cytokines activate the transcription factor NF-kappa B by stimulating the activity of a protein kinase that phosphorylates Ikappa B, an inhibitor of NF-kappa B, at sites that trigger its ubiquitination and degradation. A large, cytokine-responsive Ikappa B kinase (IKK) complex has been purified and the genes encoding 2 of its subunits have been cloned. These subunits, IKK-alpha and Ikk-beta, are protein kinases whose function is needed for NF-kappa B activation by pro-inflammatory stimuli. IKK is composed of similar amounts of IKK-alpha, Ikk-beta, which are differentially processed forms of a third subunit, IKK-gamma. Ikk-gamma interacts preferentially with IKK-beta and is required for the activation of the IKK complex.

Long Name

IkB Kinase gamma

Alternate Names

FIP3P, IKBKG, IKKAP1, NEMO

Gene Symbol

IKBKG

Additional IKK gamma Products

Product Documents for IKK gamma Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IKK gamma Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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