IKK gamma Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76799

Novus Biologicals, part of Bio-Techne
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NBP1-76799
NBP1-76799-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (94%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Flow Cytometry Control, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all IKK gamma Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid peptide near the carboxy terminus of human IKK gamma. The immunogen is located within the last 50 amino acids of IKK gamma. Amino Acid Squence: CQYQAPDMDTLQIHVME

Specificity

IKK gamma has no cross response to IKK alpha or IKK beta.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IKK gamma Antibody - BSA Free

Western Blot: IKK gamma AntibodyBSA Free [NBP1-76799]

Western Blot: IKK gamma AntibodyBSA Free [NBP1-76799]

Western Blot: IKK gamma Antibody [NBP1-76799] - Analysis of IKKg in HeLa whole cell lysate with anti-IKKg/NEMO using NBP1-76799 at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: IKK gamma Antibody - BSA Free [NBP1-76799]

Immunocytochemistry/ Immunofluorescence: IKK gamma Antibody - BSA Free [NBP1-76799]

Immunocytochemistry/Immunofluorescence: IKK gamma Antibody [NBP1-76799] - Hela cells with IKK gamma antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: IKK gamma Antibody - BSA Free [NBP1-76799]

Immunocytochemistry/ Immunofluorescence: IKK gamma Antibody - BSA Free [NBP1-76799]

Immunocytochemistry/Immunofluorescence: IKK gamma Antibody [NBP1-76799] - HeLa cells with IKK gamma antibody at 5 ug/ml.

Applications for IKK gamma Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Western Blot

1 ug/ml
Application Notes
A 52 kDa band should be detected in Western Blot. Flow-IC reactivity reported in (PMID:19015396).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IKK gamma

Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappaB is associated with IkappaB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. The IkappaB kinase (IKKalpha and IKKbeta) phosphorylates IkB and mediates NF-kappaB activation. A novel molecule in the IKK complex was recently identified and termed IKKgamma/NEMO/FIP3/IKKAP1. IKKgamma interacts with IKKbeta and is required for the activation of IKK complex and NF-kappaB by LPS, PMA, TNF, and IL-1 stimulation. FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-kappaB activation.

Long Name

IkB Kinase gamma

Alternate Names

FIP3P, IKBKG, IKKAP1, NEMO

Gene Symbol

IKBKG

UniProt

AF074382 (Human)

Additional IKK gamma Products

Product Documents for IKK gamma Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IKK gamma Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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