IL-1 RI Antibody (JAMA-147) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80794

Novus Biologicals, part of Bio-Techne
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NBP2-80794
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Catalog #
BSA Free
NB100-65865
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Block/Neutralize, CyTOF-ready, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG Clone # JAMA-147

Format

Azide and BSA Free

Concentration

1 mg/ml

View all IL-1 RI Antibodies »

Product Summary for IL-1 RI Antibody (JAMA-147) - Azide and BSA Free

Immunogen

Extracellular domain of murine IL-1R.

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Applications for IL-1 RI Antibody (JAMA-147) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunoprecipitation

1:10-1:500
Application Notes
Measured by its ability to neutralize IL-1 alpha /IL-1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.05-0.15 ug/mL in the presence of 50 pg/mL Recombinant Mouse IL-1 alpha /IL-1F1 and 1.25 ug/mL concanavalin A.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-1 RI

IL1 binds antigen CD121a, which is a transmembrane glycoprotein and a member of the immunoglobulin superfamily expressed on T cells, thymocytes, dendritic cells, fibroblasts, vascular endothelial cells, epithelial cells and neural cells. CD121a binds the mature forms of IL1 alpha, IL1 beta, and IL1 Ra proteins. High affinity binding and signaling by the type I IL1 receptor requires CD121a and the accessory protein, IL1 R AcP, which does not bind IL1 alone, but forms a dimer capable of binding IL1. Interleukin 1 (IL1), which has a role as a mediator in inflammation, consists of 2 separate but related proteins, IL1 alpha and IL1 beta. Cell surface receptors for the 2 forms of IL1 are identical. IL1R Type I contains a single membrane spanning segment, a large cytoplasmic region, and an extracellular, IL1 binding portion composed of 3 immunoglobulin like domains.

Long Name

Interleukin 1 Receptor I

Alternate Names

CD121a, IL-1RI, IL1R1, IL1RI

Gene Symbol

IL1R1

Additional IL-1 RI Products

Product Documents for IL-1 RI Antibody (JAMA-147) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IL-1 RI Antibody (JAMA-147) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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