Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

ELISA, Flow Cytometry

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 kappa Clone # 13A

Format

BSA Free

Concentration

0.5 mg/ml

View all IL-13 Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was IL13.

Reactivity Notes

This antibody is reactive to Mouse.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1 kappa

Scientific Data Images for IL-13 Antibody (13A) - BSA Free

Flow Cytometry: IL-13 Antibody (13A) - BSA Free [NBP1-43239]

Flow Cytometry: IL-13 Antibody (13A) - BSA Free [NBP1-43239]

Flow Cytometry: IL-13 Antibody (13A) [NBP1-43239] - Analysis using PE conjugate of NBP1-43239. BALB/c splenocytes were stimulated for 3 days with plate-bound Anti-Mouse CD3e Functional Grade Purified (cat. 16-0031), soluble Anti-Mouse CD28 Functional Grade Purified (cat. 16-0281), Mouse IL-2 Recombinant Protein (cat. 14-8021), and Mouse IL-4 Recombinant Protein (cat. 14-8041). Cells were then restimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) for 5 hours. Following restimulation, cells were fixed and permeabilized then stained with Anti-Mouse CD4 FITC (cat. 11-0042) and 0.125 ug of Rat IgG1 K Isotype Control PE (cat. 12-4301) or 0.125 ug of Anti-Mouse IL-13 PE. Viable cells, as determined by Fixable Viability Dye eFluor® 780, were used for analysis.

Applications for IL-13 Antibody (13A) - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The eBio13A antibody has been tested as the capture antibody in a sandwich ELISA (and ELISPOT) for analysis of mouse IL-13, in combination with the biotinylated eBio1316H antibody for detection (13-7135) and recombinant mouse IL-13 (14-8131) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1.0 - 4.0 ug/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8.0 pg/ml should be included in each ELISA plate.

The Functional Grade Purified eBio1316H antibody (16-7135) is recommended for in vitro blocking studies.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IL-13

The 13A antibody reacts with mouse IL-13. IL-13 is a cytokine produced mainly by Th2 cells, but also by antigen-primed CD8 T cells. IL-13 has a strong involvement in allergic inflammation and parasitic clearing and in cancer models has been shown to have either inhibitory or stimulatory activity depending on the tumor. In humans, IL-13 is found to play a role in isotype switching in B cells. IL-13 is implicating in down modulating macrophage activity, through the reduction of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12)

Long Name

Interleukin 13

Alternate Names

IL13, NC30, P600

Gene Symbol

IL13

Additional IL-13 Products

Product Documents for IL-13 Antibody (13A) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL-13 Antibody (13A) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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