IL-15R alpha Antibody (JM7A4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43238

Clone JM7A4 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Validated:

Flow Cytometry, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # JM7A4

Format

BSA Free

Concentration

0.5 mg/ml

View all IL-15R alpha Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was IL15Ra.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for IL-15R alpha Antibody (JM7A4) - BSA Free

Flow Cytometry: IL-15R alpha Antibody (JM7A4) - BSA Free [NBP1-43238]

Flow Cytometry: IL-15R alpha Antibody (JM7A4) - BSA Free [NBP1-43238]

Flow Cytometry: IL-15 R alpha Antibody (JM7A4) [NBP1-43238] - Staining of adherent human PBMCs stimulated with LPS and IFN gamma for 24 hours with 0.25 ug of Mouse IgG2b K Isotype Control Purified (NBP1-43317) (open histogram) or 0.25 ug of Anti-Human IL-15 Receptor Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE. Cells in the large scatter population were used for analysis.

Applications for IL-15R alpha Antibody (JM7A4) - BSA Free

Application
Recommended Usage

Flow Cytometry

<0.5 mg/10^6 cells in 100 ul

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This JM7A4 antibody has been tested by flow cytometric analysis of LPS and IFN-γ-stimulated PBMCs. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IL-15R alpha

The JM7A4 monoclonal antibody reacts with the IL-15 receptor alpha chain (IL-15Ralpha). IL-15 and IL-2 are cytokines with overlapping, but distinct, biologic effects. Their receptors share 2 subunits, the IL2R-beta and -gamma chains, which are essential for signal transduction. The IL-15R-alpha receptor is structurally related to IL-2R-alpha. However, the IL-15Ralpha alone binds IL-15 with a 1,000-fold higher affinity than that seen with IL-2R-alpha and IL-2. In the human, 3 differentially spliced human IL15R-alpha variants that are all capable of high affinity binding of IL-15 have been isolated. The cytoplasmic domain of IL15R-alpha, like that of IL2R-alpha, is dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high affinity binding. At high concentrations, IL-15, like IL-2, is able to signal through a complex of IL2R-beta and IL2R-gamma in the absence of the alpha subunit.

Long Name

Interleukin 15 Receptor alpha

Alternate Names

CD215, IL-15 R alpha, IL-15Ra, IL15 R alpha, IL15RA

Entrez Gene IDs

3601 (Human)

Gene Symbol

IL15RA

UniProt

Q13261

Additional IL-15R alpha Products

Product Documents for IL-15R alpha Antibody (JM7A4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IL-15R alpha Antibody (JM7A4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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