IL-16 Antibody (14.1) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09027
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Cited:
ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 14.1
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The antibody was raised against IL-16 using a synthetic peptide corresponding to the predicted amino acid sequence of a hydrophilic region of IL-16, conjugated to keyhole limpet hemocyanin.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for IL-16 Antibody (14.1) - Chimeric - Azide and BSA Free
![Flow Cytometry: IL-16 Antibody (14.1) - Chimeric - Azide and BSA Free [NBP3-09027]](https://resources.bio-techne.com/images/products/IL-16-Antibody-14-1-Chimeric-Flow-Cytometry-NBP3-09027-img0001.jpg "Flow Cytometry: IL-16 Antibody (14.1) - Chimeric - Azide and BSA Free [NBP3-09027]")
Flow Cytometry: IL-16 Antibody (14.1) - Chimeric - Azide and BSA Free [NBP3-09027]
Flow Cytometry: IL-16 Antibody (14.1) - Chimeric [NBP3-09027] - Human peripheral blood leukocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 14.1 (NBP3-09027, pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with NBP3-09027. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.Applications for IL-16 Antibody (14.1) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
The antibody has been used in Western blot analysis to detect IL-16. It has been used in ELISA-type binding assays. The antibody has been used to stain endogenous IL-16 in sheets from murine ear skin after intradermal injection of TNF- alpha , and in flow cytometry to detect IL-16 on epidermal cells and emigrated cutaneous DC (Stoitzner et al, 2001).
The antibody has been used in Western blot analysis to detect IL-16. It has been used in ELISA-type binding assays. The antibody has been used to stain endogenous IL-16 in sheets from murine ear skin after intradermal injection of TNF- alpha , and in flow cytometry to detect IL-16 on epidermal cells and emigrated cutaneous DC (Stoitzner et al, 2001).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IL-16
Long Name
Interleukin 16
Alternate Names
IL16, LCF
Gene Symbol
IL16
Additional IL-16 Products
Product Documents for IL-16 Antibody (14.1) - Chimeric - Azide and BSA Free
Product Specific Notices for IL-16 Antibody (14.1) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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