IL-16 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21240

Novus Biologicals, part of Bio-Techne
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NBP3-21240-100ul
NBP3-21240-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IL-16 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QRARSFPLTRSQSCETKLLDEKTSKLYSISSQVSSAVMKSLLCLPSSISCAQTPCIPKEGASPTSSSNEDSAANGSAETSALDTGFSLNLSELREYTEGLTEAKEDDDG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IL-16 Antibody

Immunocytochemistry/Immunofluorescence: IL-16 Antibody [NBP3-21240] -

Immunocytochemistry/Immunofluorescence: IL-16 Antibody [NBP3-21240] -

Staining of human cell line U-2 OS shows positivity in nucleus, plasma membrane & cytoplasm.
Immunohistochemistry-Paraffin: IL-16 Antibody [NBP3-21240] -

Immunohistochemistry-Paraffin: IL-16 Antibody [NBP3-21240] -

Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: IL-16 Antibody [NBP3-21240] -

Immunohistochemistry-Paraffin: IL-16 Antibody [NBP3-21240] -

Analysis in human lymph node and cerebral cortex tissues using Anti-IL16 antibody. Corresponding IL16 RNA-seq data are presented for the same tissues.
View 1 more images

Applications for IL-16 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-16

Interleukin-16 (IL-16, also known as lymphocyte chemoattractant factor (LCF)) is a CD8+ T cell-derived cytokine. It acts as a chemoattractant for CD4+ T-cells, monocytes and eosinophils. IL-16 has also been shown to suppress HIV-1 replication in vitro. It is expressed as a precursor molecule (pro-IL-16) in various tissues including spleen, thymus, lymph nodes, peripheral leukocytes, bone marrow and cerebellum. The active form is assumed to be a proteolytic cleavage product of pro-IL-16 existing, under physiological conditions, predominantly as a noncovalently linked multimer. The sequence and structure of IL-16 is conserved across species, human and murine IL-16 show significant cross-species reactivity.

Long Name

Interleukin 16

Alternate Names

IL16, LCF

Gene Symbol

IL16

Additional IL-16 Products

Product Documents for IL-16 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IL-16 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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