IL-17/IL-17A Antibody (4H1524) - Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25229

Novus Biologicals, part of Bio-Techne
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NBP2-25229
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Block/Neutralize, CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4H1524

Format

Azide Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IL-17/IL-17A Antibodies »

Product Summary for IL-17/IL-17A Antibody (4H1524) - Azide Free

Immunogen

A portion of amino acids 1-100 was used as immunogen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for IL-17/IL-17A Antibody (4H1524) - Azide Free

Western Blot: IL-17/IL-17A Antibody (4H1524)Azide Free [NBP2-25229]

Western Blot: IL-17/IL-17A Antibody (4H1524)Azide Free [NBP2-25229]

Western Blot: IL-17/IL-17A Antibody (4H1524) - Azide Free [NBP2-25229] - Analysis of IL-17A in (1) human, (2) mouse and (3) rat full-length recombinant IL-17A protein using NBP2-25229 at 1 ug/ml.
Flow Cytometry: IL-17/IL-17A Antibody (4H1524) - Azide Free [NBP2-25229]

Flow Cytometry: IL-17/IL-17A Antibody (4H1524) - Azide Free [NBP2-25229]

Flow Cytometry: IL-17/IL-17A Antibody (4H1524) - Azide Free [NBP2-25229] - Analysis using the PE conjugate of NBP2-25229. IL-17A positive cells in PMA/IM stimulated fresh PBMC: PBMC stimulated for 6h with PMA (25ng/ml) & IM (250ng/ml), in the presence of BFA. Cells were surface stained with CD4 APC, fixed & permeabilizedfor intracellular staining with PE conjugated
Block/Neutralize: IL-17/IL-17A Antibody (4H1524) - Azide Free [NBP2-25229] - Neutralization by Human IL-17A Antibody. Secretion of IL-6 by 3T3 cells stimulated with recombinant human IL-17A (20ng/ml) is neutralized by increasing concentrations of IL-17A monoclonal antibody. Antibody at different concentrations was added to cells 1hr prior to addition of cytokine. Supernatant collected from 60hr cultures was used for analysis to measure secreted IL-6 by ELISA.

Applications for IL-17/IL-17A Antibody (4H1524) - Azide Free

Application
Recommended Usage

Flow Cytometry

1ul/1 million cells

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 ml sterile PBS

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-17/IL-17A

Interleukin 17 (IL-17) is a pro-inflammatory cytokine produced by a subset of T helper cells that develops distinct from the Th1- and Th2- cell differentiation pathways. IL-17, also known as CTLA-8, stimulates induction of other pro-inflammatory cytokines TNF alpha, IL-1 beta, IL-6, and IL-8, and reports strongly suggest the involvement of IL-17 in several chronic inflammatory diseases such as rheumatoid arthritis, psoriasis and multiple sclerosis. TGF-Beta (differentiation) and IL-23 (expansion) are required for induction and maintenance of Th17 (IL-17 producing) cells, which in turn induce the other pro-inflammatory cytokines. IL-17 (~32kDa) protein is produced and exists as a homo-dimer, has homology to a herpes virus early protein, is one of the six members (IL-17A-F) of this cytokine family, and is well characterzed and highly expressed by activated effector memory T cells.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Gene Symbol

IL17A

Additional IL-17/IL-17A Products

Product Documents for IL-17/IL-17A Antibody (4H1524) - Azide Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IL-17/IL-17A Antibody (4H1524) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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