IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72027
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # TC11-18H10
Format
Azide and BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
E. coli-expressed, recombinant mouse IL-17/IL-17A
Specificity
Recognizes mouse Interleukin 17, a 32kDa disulfide-linked homodimer consisting of two 136 amino acid chains, which belong to the IL-17 family (classified as IL-17A to IL-17F), and signal through the IL-17 receptor (IL-17R/CDw217).
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free
Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free [NBP1-72027]
Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free [NBP1-72027] - IL-17/IL-17A Antibody (TC11-18H10) [NBP1-72027] - Treatment with HBOT or PFD decreased IL-17A expression in the AD lesions. IHC for IL-17A on mouse skin. Scale bar is 100 um. The number of IL-17A-expressing cells was counted in five high-power fields (HPF) chosen at random in each slide by three different pathologists. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0109297) licensed under a CC-BY license.Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free [NBP1-72027]
Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (TC11-18H10) [NBP1-72027] - Analysis of anti-IL-17 antibody of mouse spleen at concentration 10 ug/ml.Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free [NBP1-72027] -
Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free [NBP1-72027] - TG2-dependent chemokine production in keratinocytes is critical for IL-17-producing CCR6+ gamma deltaT-cell & neutrophil dermal infiltration.a, b Effect of DMSO- or IMQ-treated WT- or TG2−/−-CM on CCR6+ gamma deltaT-cell (a) & CD11b+ Ly-6G+ cell (b) migration according to Transwell migration assays. Flow cytometric analysis of the percentage of cells migrating toward the CM in the presence or absence of anti-CXCL1 (a) & anti-CCL20 (b) antibodies (n = 3/group). c, d WT & TG2−/− mouse-derived CCR6+ gamma deltaT-cell (c) & CD11b+ Ly-6G+ cell (d) migration in response to recombinant mouse CCL20 & CXCL1, respectively. Data represent the mean ± SEM (n = 3/group). *p < 0.05; **p < 0.01. e, f Cells were isolated from the back skin of WT & TG2−/− mice treated with IMQ for four consecutive days & analyzed by flow cytometry. Representative flow cytometric profiles of CCR6+ gamma deltaTCRlow cells (e) & CD11bhigh Ly-6G+ cells (f). The percentage of cells is shown. Data represent the mean ± SEM (n = 9/group). g Skin sections were immunostained with CCR6 (green)- & IL-17 (red)-specific antibodies. Nuclei were stained with DAPI (blue). Scale bar, 100 μm. h–j Back skin was separated into epidermis & dermis & analyzed by RT-PCR for IL-17-producing gamma deltaT-cell markers (h) (Ccr6 & Il23r), IL17 & related cytokines (i) (Il17a, Il17f, & Il22), & myeloid cell-derived cytokines (j) (Il12a & Il23a). Data represent the mean ± SEM (n = 11/group). *p < 0.05; **p < 0.01 vs. WT mice. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32355189), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Flow Cytometry
1:10-1:1000
Immunohistochemistry
10 ug/ml
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
1:100-1:2000
Application Notes
Use in IHC reported in scientific literature (PMID 27069818)..
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
BBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Background: IL-17/IL-17A
Long Name
Interleukin 17
Alternate Names
CTLA-8, CTLA8, IL-17A, IL17, IL17A
Entrez Gene IDs
16171 (Mouse)
Gene Symbol
IL17A
UniProt
Additional IL-17/IL-17A Products
Product Documents for IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free
Product Specific Notices for IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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