IL-23A/IL-23 P19 Antibody (eBio473P19 (eBio11P19, eBio25P19)) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43237

Novus Biologicals, part of Bio-Techne
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NBP1-43237-0.025mg
NBP1-43237-0.5mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # eBio473P19 (eBio11P19, eBio25P19)

Format

BSA Free

Concentration

0.5 mg/ml

View all IL-23 Antibodies »

Product Summary

Immunogen

The immunogen for this antibody was IL23 p19.

Reactivity Notes

This antibody is reactive to Human.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

473P19 is a cocktail of the two clones, 11P19 and 25P19 at 0.5mg/mL each.

Applications

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

2 ug/ml
Application Notes
The 473P19 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-23 (p19p40) protein levels in combination with the biotinylated (p40-specific) C8.6 antibody (NBP1-48601B) for detection and recombinant human IL-23 (NBP1-48602) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 2.5-8.0 ug/ml. A standard curve consisting of doubling dilutions of the recombinant IL-23 standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate. TMB, rather than ABTS, should be used as the substrate.The antibody 473p19 also works in immunoblotting under both reducing and nonreducing conditions at 2ug/ml.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IL-23

The 473P19 antibody reacts with the p19 subunit of human IL-23. The 473P19 antibody was generated from immunization with authentic, insect cell-expressed, recombinant human IL-23 heterodimer. The use of a p19-specific capture antibody and a p40-specific detection antibody yields a human IL-23 sandwich ELISA exquisitely specific for human IL-23. IL-12 p40 monomer and IL-12 p70 were run in the assay at 200 ng/ml with no interference or cross-reactivity observed. A panel of 20 unrelated cytokines was also run in the IL-23 ELISA at 100 ng/ml with no cross reactivity observed. The assay has been validated by specific detection of significant levels of native human IL-23 protein in supernatants from a variety of different activated dendritic cell populations. IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-g. Mouse IL-23 does induce strong proliferation of memory T cells (but not nave T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12 human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-g production by nave and memory T cells, as compared to IL-12. IL-23-dependent, IL-17-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-g and IL-4, have each been found to negatively regulate the generation of these Th-17 cells. More recently, de novo differentiation of Th-17 cells in the absence of IL-23 has been demonstrated by treatment of nave CD4 cells with TGF-beta1 and IL-6.

Long Name

Interleukin 23

Alternate Names

IL-23 p19/IL-12 p40, IL-23p19/IL-12p40, IL23, SGRF

Entrez Gene IDs

51561 (Human)

Gene Symbol

IL23A

UniProt

Q9NPF7

Additional IL-23 Products

Product Documents

Product Datasheets

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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