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IL-23A/IL-23 P19 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77257

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Equine (93%), Guinea Pig (93%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of mature human IL-23. The immunogen is located the last 50 amino acids of IL-23.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-23A/IL-23 P19 Antibody - BSA Free

Western Blot: IL-23A/IL-23 P19 AntibodyBSA Free [NBP1-77257]

Western Blot: IL-23A/IL-23 P19 AntibodyBSA Free [NBP1-77257]

Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Increased cytokine expression in N-WASP-null skin. Representative immunoblots and quantification of indicated proteins from epidermal lysates of 7-8-week old con and ko mice (n: 4/4, mean?+-?SD, two-tailed unpaired t-test). Representative immunoblots and quantification of indicated proteins of co-precipitations of IL-23p19 with IL-12p40 from epidermal lysates of 7-8-week-old con and ko mice (n: 4/4, mean?+-?SD, two-tailed unpaired t-test; *p?less than or equal ?0.05; **p?less than or equal ?0.01; ***p?less than or equal ?0.001) Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03704-z) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257]

Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257]

Immunocytochemistry/Immunofluorescence: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Immunofluorescence of IL-23 in Raji cells with IL-23 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257]

Immunohistochemistry-Paraffin: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257]

Immunohistochemistry-Paraffin: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Abdominal Aortic Aneurysm (AAA) Tissues (Validation of IL-23 in Human Abdominal Aortic Aneurysm (AAA) Tissues

Applications for IL-23A/IL-23 P19 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/mL

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-4 ug/ml
Application Notes
Use in immunoprecipitation reported in scientific literature (PMID: 29650963).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-23

Like interleukin-27 (IL-27), IL-23 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the IL-12p40 subunit and a novel p19 subunit. IL-23 is secreted by activated dendritic cells, macrophages, and monocytes. Its biological activities include enhancing the proliferation of memory T cells and the production of IFN-gamma, IL-12, and TNF-alpha from activated T cells, and can stimulate macrophages to produce TNF-alpha and nitric oxide. It has also been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer.

Long Name

Interleukin 23

Alternate Names

IL-23 p19/IL-12 p40, IL-23p19/IL-12p40, IL23, SGRF

Gene Symbol

IL23A

Additional IL-23 Products

Product Documents for IL-23A/IL-23 P19 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-23A/IL-23 P19 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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