IL-23A/IL-23 P19 Antibody - BSA Free

Western Blot: IL-23A/IL-23 P19 AntibodyBSA Free [NBP1-77257]

Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Loading: 30 ng of human IL-23 recombinant protein.Antibodies: IL-23 (4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: IL-23A/IL-23 P19 AntibodyBSA Free [NBP1-77257]

Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Loading: 20ug of lysates per lane.Antibodies: IL-23, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257]

Immunohistochemistry: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Staining of human pancreas tissue with antibody at 5 ug/mL.

Western Blot: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] -

Western Blot: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Increased cytokine expression in N-WASP-null skin. a qRT-PCR analysis of indicated genes from back skin RNA of con & ko at indicated ages (n > = 3/3, two-tailed unpaired t-test). All normalized to GAPDH. b Representative toluidine blue staining for barrier defect in con & ko mice at indicated ages (P: E15.5 embryos used as positive controls; 2 days (2d) n: 6/5, 6 days(6d) n: 4/5, 3 independent experiments). c Representative immunofluorescence staining & quantification of IL-23p19 of con & ko back skin at indicated ages (n: 3/3, two-tailed paired t-test). Scale bar: 100 µm. d Representative sorting strategy of CD45+, alpha6H, & alpha6L cells from con & ko epidermal cells & qRT-PCR analysis of IL-23A & IL-17A expression in sorted cells (n: 3/3, mean ± SD, two-tailed unpaired t-test, 3 independent sortings). e Representative immunoblots & quantification of indicated proteins from epidermal lysates of 7–8-week old con & ko mice (n: 4/4, mean ± SD, two-tailed unpaired t-test). f Representative immunoblots & quantification of indicated proteins of co-precipitations of IL-23p19 with IL-12p40 from epidermal lysates of 7–8-week-old con & ko mice (n: 4/4, mean ± SD, two-tailed unpaired t-test; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001) Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-018-03704-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] -

Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation of IL-23A/IL-23 P19 in Raji Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Raji Cells labeling IL-23A/IL-23 P19 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] -

Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation of IL-23A/IL-23 P19 in Raji Cells. Immunocytochemical analysis of Raji cells using anti-IL-23A/IL-23 P19 antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] -

Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation of IL-23A/IL-23 P19 in Human Pancreas Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling IL-23A/IL-23 P19 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Western Blot: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] -

Western Blot: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation in Human Raji Cells. Loading: 15 ug of lysates per lane.Antibodies: IL-23A/IL-23 P19 (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Absence of blocking peptideLane B: Presence of blocking peptide.