IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80807

Novus Biologicals, part of Bio-Techne
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NBP2-80807
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Conjugate
Catalog #
Alexa Fluor 350
NBP2-19015AF350
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Alexa Fluor 405
NBP2-19015AF405
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Alexa Fluor 488
NBP2-19015AF488
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Alexa Fluor 532
NBP2-19015AF532
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Alexa Fluor 594
NBP2-19015AF594
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Alexa Fluor 647
NBP2-19015AF647
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Alexa Fluor 700
NBP2-19015AF700
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Alexa Fluor 750
NBP2-19015AF750
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Allophycocyanin
NBP2-19015APC
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Allophycocyanin/Cy7
NBP2-19015APCCY7
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Biotin
NBP2-19015B
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CoraFluor 1
NBP2-19015CL1
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DyLight 350
NBP2-19015UV
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DyLight 405
NBP2-19015V
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DyLight 488
NBP2-19015G
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DyLight 550
NBP2-19015R
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DyLight 594
NBP2-19015DL594
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DyLight 650
NBP2-19015C
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DyLight 680
NBP2-19015FR
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DyLight 755
NBP2-19015IR
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FITC
NBP2-19015F
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HRP
NBP2-19015H
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Janelia Fluor 525
NBP2-19015JF525
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Janelia Fluor 549
NBP2-19015JF549
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Janelia Fluor 585
NBP2-19015JF585
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Janelia Fluor 635
NBP2-19015JF635
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Janelia Fluor 646
NBP2-19015JF646
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Janelia Fluor 669
NBP2-19015JF669
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mFluor Violet 450 SE
NBP2-19015MFV450
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mFluor Violet 500 SE
NBP2-19015MFV500
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mFluor Violet 610 SE
NBP2-19015MFV610
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PE
NBP2-19015PE
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PE/Atto594
NBP2-19015PEATT594
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PE/Cy5.5
NBP2-19015PECY55
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PE/Cy7
NBP2-19015PECY7
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PerCP
NBP2-19015PCP
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Forumulation
Catalog #
BSA Free
NBP2-19015
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 34N4G11

Format

Azide and BSA Free

Concentration

1 mg/ml

View all IL-27 R alpha/WSX-1/TCCR Antibodies »

Product Summary for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free

Immunogen

A portion of amino acids 200-250 of human IL-27R (WSX-1) was used as the immunogen for this antibody.

Localization

Membrane (with Extracellular and Cytoplasmic domains)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free

Immunohistochemistry: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free [NBP2-80807]

Immunohistochemistry: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free [NBP2-80807]

Immunohistochemistry: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free [NBP2-80807] - IHC-P detection of IL27RA protein in a section of normal human colon tissue using IL27RA antibody (34N4G11) at a concentration of 5ug/ml. Intense membrane with cytoplasmic staining was observed in the cells of crypts and the absorptive columnar epithelium
Flow (Cell Surface): IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free [NBP2-80807]

Flow (Cell Surface): IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free [NBP2-80807]

Flow (Cell Surface): IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free [NBP2-80807] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-19015. Surface staining of IL27R on freshly isolated (PPI negative) human PBMCs using IL-27R antibody and CD3 antibody at 10 ul/10^6 cells. Flow kit, mouse IgG2b isotype control antibody, and RBC lysis buffer were used for this test.
Immunohistochemistry: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free [NBP2-80807]

Immunohistochemistry: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free [NBP2-80807]

Immunohistochemistry: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free [NBP2-80807] - Adenocarcinoma of the stomach probed with IL-27R antibody at 5 ug/ml. Image from the standard format of this antibody.
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Applications for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free

Application
Recommended Usage

Flow (Cell Surface)

1 ug/ml

Flow Cytometry

1 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-27 R alpha/WSX-1/TCCR

The IL-27 Receptor complex includes WSX-1 and gp130. IL-27R (WSX-1) is 65-70 kDa protein and is highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes including CD4+ and CD8+ T cells, monocytes, DCs and NK cells (1-3). IL-27R activation includes STAT1, STAT3, STAT4 and STAT5, leading to differing pathway dependent effector activities. Like the IL12/IL-12R complex, the IL-27/IL-27R complex also has Th1 enhancing activity especially enhancement of IFNg production by naive T cells and NK cells (1). IL-27R mediates downregulation of Th2 activity by findings that IL-27R deficiencies mitigate inflammatory pathology in helminthic infections in mice (1, 4, 5). The possibility of utilizing IL-27R as a target for autoimmune inflammatory diseases, especially of mucosal surfaces as in human IBD, is under investigation, measured by Th2 type cytokines and bolstered through Th2

Long Name

Interleukin-27 Receptor Subunit alpha

Alternate Names

IL-27 R alpha, IL-27Ra, IL27R alpha, IL27RA, TCCR, WSX-1

Gene Symbol

IL27RA

Additional IL-27 R alpha/WSX-1/TCCR Products

Product Documents for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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