IL-27R (WSX-1) is 65-70 kDa protein and is highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes including CD4+ and CD8+ T cells, monocytes, DCs and NK cells

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - IHC-P detection of IL27RA protein in a section of human renal/kidney cancer tissue using IL27RA antibody (34N4G11) at a concentration of 5ug/ml. The representative image shows membrane-cytoplasmic positivity for IL27RA protein in the cancer cells.

Flow (Cell Surface): IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Flow (Cell Surface): IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-19015. Surface staining of IL27R on freshly isolated (PPI negative) human PBMCs using IL-27R antibody and CD3 antibody at 10 ul/10^6 cells. Flow kit, mouse IgG2b isotype control antibody, and RBC lysis buffer were used for this test.

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - Adenocarcinoma of the stomach probed with IL-27R antibody at 5 ug/ml.

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - FFPE human prostate probed with IL-27R antibody at 5 ug/ml.

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - IHC-P detection of IL27RA protein in a section of normal human colon tissue using IL27RA antibody (34N4G11) at a concentration of 5ug/ml. Intense membrane with cytoplasmic staining was observed in the cells of crypts and the absorptive columnar epithelium.

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - IHC-P detection of IL27RA protein in a section of human colo-rectal cancer tissue using IL27RA antibody (34N4G11) at a concentration of 5ug/ml. The cancer cells showed specific positivity for IL27RA and the staining intensity was more towards the cellular membranes. The tumor stroma was largely negative for staining.

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - IHC-P detection of IL27RA protein in a section of human stomach cancer tissue using IL27RA antibody (34N4G11) at a concentration of 5ug/ml. The cancer cells showed intense membrane-cytoplasmic positivity for IL27RA protein, whereas, the glands in adjacent area and the associated stroma developed distinctly weak staining.

Flow Cytometry: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Flow Cytometry: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - Surface staining on human peripheral blood monocytes using IL-27R antibody at 1 ug/10^6 cells (red represents specific antibody and green represents isotype control). APC-conjugated goat anti-mouse Ig F(ab')2 secondary antibody , CS flow kit and an FcR block were used for this test.

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Applications for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free

Application

Recommended Usage

Flow (Cell Surface)

1 ug/ml

Flow Cytometry

1 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Application Notes

This antibody is Cytof ready.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-27 R alpha/WSX-1/TCCR

The IL-27 Receptor complex includes WSX-1 and gp130. IL-27R (WSX-1) is 65-70 kDa protein and is highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes including CD4+ and CD8+ T cells, monocytes, DCs and NK cells (1-3). IL-27R activation includes STAT1, STAT3, STAT4 and STAT5, leading to differing pathway dependent effector activities. Like the IL12/IL-12R complex, the IL-27/IL-27R complex also has Th1 enhancing activity especially enhancement of IFNg production by naive T cells and NK cells (1). IL-27R mediates downregulation of Th2 activity by findings that IL-27R deficiencies mitigate inflammatory pathology in helminthic infections in mice (1, 4, 5). The possibility of utilizing IL-27R as a target for autoimmune inflammatory diseases, especially of mucosal surfaces as in human IBD, is under investigation, measured by Th2 type cytokines and bolstered through Th2

Long Name

Interleukin-27 Receptor Subunit alpha

Alternate Names

IL-27 R alpha, IL-27Ra, IL27R alpha, IL27RA, TCCR, WSX-1

Entrez Gene IDs

9466 (Human)

Gene Symbol

IL27RA

OMIM

605350 (Human)

UniProt

Q6UWB1

Additional IL-27 R alpha/WSX-1/TCCR Products

Product Documents for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19015

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Format

Concentration

View all IL-27 R alpha/WSX-1/TCCR Antibodies »

Product Specifications

Immunogen

Localization

Specificity

Clonality

Host

Isotype

Scientific Data Images for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Flow (Cell Surface): IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Immunohistochemistry-Paraffin: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Flow Cytometry: IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free [NBP2-19015]

Applications for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free

Flow (Cell Surface)

Flow Cytometry

Immunohistochemistry

Immunohistochemistry-Paraffin

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: IL-27 R alpha/WSX-1/TCCR

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

OMIM

UniProt

Additional IL-27 R alpha/WSX-1/TCCR Products

Product Documents for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for IL-27R alpha/WSX-1/TCCR Antibody (34N4G11) - BSA Free