IL-3 Antibody (OTI3B11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47806

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3B11

Concentration

1 mg/ml

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Product Summary for IL-3 Antibody (OTI3B11)

Immunogen

Human recombinant protein fragment corresponding to amino acids 20-152 of human IL-3 (NP_000579) produced in E.coli.

Specificity

This antibody is specific for Homo sapiens interleukin 3 (colony-stimulating factor, multiple) (IL3).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-3 Antibody (OTI3B11)

Western Blot: IL-3 Antibody (OTI3B11) [NBP1-47806]

Western Blot: IL-3 Antibody (OTI3B11) [NBP1-47806]

Western Blot: IL-3 Antibody (3B11) [NBP1-47806] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL3.
Immunocytochemistry/ Immunofluorescence: IL-3 Antibody (OTI3B11) [NBP1-47806]

Immunocytochemistry/ Immunofluorescence: IL-3 Antibody (OTI3B11) [NBP1-47806]

Immunocytochemistry/Immunofluorescence: IL-3 Antibody (3B11) [NBP1-47806] - Staining of A549 cells using anti-IL3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]

Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]

Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue.
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Applications for IL-3 Antibody (OTI3B11)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IL-3

The protein encoded by IL-3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders

Long Name

Interleukin 3

Alternate Names

IL3, MCGF, MULTI-CSF

Entrez Gene IDs

3562 (Human)

Gene Symbol

IL3

Additional IL-3 Products

Product Documents for IL-3 Antibody (OTI3B11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IL-3 Antibody (OTI3B11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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