IL-32 alpha Antibody (12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06577

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IL-32 alpha Antibodies »

Product Specifications

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-32 alpha (Accession#: NP_001012651.1; Met1-Lys131).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for IL-32 alpha Antibody (12)

Western Blot: IL-32 alpha Antibody (12) [NBP3-06577]

Western Blot: IL-32 alpha Antibody (12) [NBP3-06577]

Western Blot: IL-32 alpha Antibody (12) [NBP3-06577] - Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:26 kDaObserved band size:30 kDa(We are unsure as to the identity of these extra bands.)

Applications for IL-32 alpha Antibody (12)

Application
Recommended Usage

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-32 alpha

IL-32 alpha is the shortest and most abundant of four potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4) with a predicted unmodified size of 15 kDa. Potential modifications include myristoylation and N-glycosylation. Transfected IL-32 alpha was more likely to be cell-associated as compared to IL-32 beta, suggesting an intracellular function. IL-32 is induced by mitogens in peripheral lymphocytes, by IFN-gamma in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn induces cytokine expression. No ortholog has been found in mouse.

Long Name

Interleukin 32 alpha

Alternate Names

IL32 alpha

Gene Symbol

IL32

Additional IL-32 alpha Products

Product Documents for IL-32 alpha Antibody (12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IL-32 alpha Antibody (12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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