IL-32 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76684
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
ELISA, Flow Cytometry, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human IL-32. The immunogen is located within the last 50 amino acids of IL-32. Amino Acid Sequence: YGAPRGDKEELTPQK
Specificity
This antibody detects all isoforms of IL-32. Human IL-32 has 7 isoforms, including isoform 1 (234aa, 27 kDa), isoform 2 (188aa, 22 kDa), isoform 3 (178aa, 21 kDa), isoform 4 (131aa, 15 kDa), isoform 5 (168aa, 19 kDa), isoform 6 (179aa, 21 kDa) and isoform 7 (148aa, 17 kDa). NBP1-76684 can detect all human isoforms.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IL-32 Antibody - BSA Free
Western Blot: IL-32 Antibody - BSA Free [NBP1-76684] -
Western Blot: IL-32 Antibody - BSA Free [NBP1-76684] - Figure 1 Western Blot Validation with Recombinant ProteinLoading: 30 ng of human IL-32 recombinant protein per lane.Antibodies: IL-32, (Lane 1: 0.5 u/mL, Lane 2: 1 u/mL, Lane 3: 2 u/mL and Lane 4: 4 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 17kD.
Immunohistochemistry: IL-32 Antibody - BSA Free [NBP1-76684] -
Immunohistochemistry: IL-32 Antibody - BSA Free [NBP1-76684] - Validation of IL-32 in Human Spleen Tissue.Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-32 antibody at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence: IL-32 Antibody - BSA Free [NBP1-76684] -
Immunocytochemistry/ Immunofluorescence: IL-32 Antibody - BSA Free [NBP1-76684] - Figure 4 Immunofluorescence Validation of IL-32 in Human Spleen CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed human spleen cells labeling IL-32 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).Applications for IL-32 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunofluorescence
20 ug/mL
Immunohistochemistry
2-10 ug/mL
Immunohistochemistry-Paraffin
5 ug/mL
Western Blot
0.5 - 4 ug/mL
Application Notes
Use in FLOW reported in scientific literature (PMID: 30641867).
Reviewed Applications
Read 1 review rated 2 using NBP1-76684 in the following applications:
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IL-32
Long Name
Interleukin 32
Alternate Names
IL32, NK4, TAIF
Entrez Gene IDs
9235 (Human)
Gene Symbol
IL32
UniProt
Additional IL-32 Products
Product Documents for IL-32 Antibody - BSA Free
Product Specific Notices for IL-32 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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