IL-32 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82560

Novus Biologicals, part of Bio-Techne
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NBP1-82560
NBP1-82560-25ul
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IL-32 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FPKVLSDDMKKLKARMHQAIERFYDKMQNAESGRGQVMSSLAELEDDFKEGYLETVAAYYEEQHPELTPLLEKERDGLRCR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IL-32 Antibody

Western Blot: IL-32 Antibody [NBP1-82560]

Western Blot: IL-32 Antibody [NBP1-82560]

Western Blot: IL-32 Antibody [NBP1-82560] - Analysis in control (vector only transfected HEK293T lysate) and IL32 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: IL-32 Antibody [NBP1-82560]

Immunohistochemistry-Paraffin: IL-32 Antibody [NBP1-82560]

Immunohistochemistry-Paraffin: IL-32 Antibody [NBP1-82560] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in non - germinal center cells.
Immunohistochemistry-Paraffin: IL-32 Antibody [NBP1-82560]

Immunohistochemistry-Paraffin: IL-32 Antibody [NBP1-82560]

Immunohistochemistry-Paraffin: IL-32 Antibody [NBP1-82560] - Staining of human lymph node shows distinct cytoplasmic positivity in subsets of lymphoid cells.
View 3 more images

Applications for IL-32 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 25100201). For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-32

IL32 alfa is the shortest of the 4 isotypes of IL32 known, it is 134 amino acids ( kDa) protein, the IL32B (beta) is 188, IL32C is 168 and IL32D is 179 amino acids long proteins. Each subtype has an N-terminal segment and four kringle domains (NK4), that interact with several other proteins including the hepatic growth factor via c-met a tyrosine receptor kinase (5). IL-32 synergized with the intracellular nuclear oligomerization domain receptors (NOD1- and NOD2-specific muropeptides of peptidoglycans for the release of IL-1beta and IL-6. In contrast, IL-32 did not influence the cytokine production induced via TLRs (6). The anti-IL32 selective antibodies were made against an epitope that lies near the C-terminal end of the protein. The antibodies to IL32 are affinity purified on immobilized affinity based chromatography and characterized for applications in ELISA and Western blotting. The antiIL32 antibodies recognize a single band of IL32 in PC-IL32 samples. The IL32 antibodies do not cross react with other pro-inflammatory or anti-inflammatory interleukins, has also produced antibodies to other interleukins, interleukin receptors, cytokines and their receptors.

Long Name

Interleukin 32

Alternate Names

IL32, NK4, TAIF

Gene Symbol

IL32

Additional IL-32 Products

Product Documents for IL-32 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IL-32 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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