IL-33 Antibody (6H617) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27333

Novus Biologicals, part of Bio-Techne
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NBP2-27333SS
NBP2-27333
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6H617

Format

BSA Free

Concentration

1.0 mg/ml

View all IL-33 Antibodies »

Product Specifications

Immunogen

Full length recombinant protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for IL-33 Antibody (6H617) - BSA Free

Western Blot: IL-33 Antibody (6H617)BSA Free [NBP2-27333]

Western Blot: IL-33 Antibody (6H617)BSA Free [NBP2-27333]

Western Blot: IL-33 Antibody (6H617) [NBP2-27333] - Analysis of IL-33 using IL-33 monoclonal antibodyin A) HUVEC cell lysate at 10 ug/mL and B) partial recombinant IL-33 (amino acids 112-270) at 1 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Flow Cytometry: IL-33 Antibody (6H617) - BSA Free [NBP2-27333]

Flow Cytometry: IL-33 Antibody (6H617) - BSA Free [NBP2-27333]

Flow Cytometry: IL-33 Antibody (6H617) [NBP2-27333] - An intracellular stain was performed on Jurkat cells with IL-33 (6H617) antibody NBP2-27333PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Flow Cytometry: IL-33 Antibody (6H617) - BSA Free [NBP2-27333]

Flow Cytometry: IL-33 Antibody (6H617) - BSA Free [NBP2-27333]

Flow Cytometry: IL-33 Antibody (6H617) [NBP2-27333] - Analysis of IL-33. Jurkat cells (0.5 x 10^6) were intracellularly stained with 0.25 ug of mouse IgG1 isotype control (green) or purified IL-33 antibody (Clone 6H617; red). This antibody and an anti-mouse IgG PE conjugated secondary antibody were used.
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Applications for IL-33 Antibody (6H617) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.25 ug/0.5x10^6 cells

Western Blot

1 - 10 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-33

IL-33 is a 270 amino acid highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. It is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAk1, IRAk4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. It is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta.

Long Name

Interleukin 33

Alternate Names

C9orf26, DVS27, IL33, NF-HEV

Gene Symbol

IL33

UniProt

O95760

Additional IL-33 Products

Product Documents for IL-33 Antibody (6H617) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IL-33 Antibody (6H617) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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