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IL-34 Antibody (C6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-41879

Novus Biologicals, part of Bio-Techne
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NBP3-41879

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # C6

Concentration

1 mg/ml

Product Specifications

Immunogen

Immunized with recombinant Human IL-34 (Uniprot#: Q6ZMJ4; Met1-Pro242 (Uniprot#: Q6ZMJ4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for IL-34 Antibody (C6)

IL-34 Antibody (C6)

Western Blot: IL-34 Antibody (C6) [NBP3-41879] -

Western Blot: IL-34 Antibody (C6) [NBP3-41879] - Sample: Lane1: Rat Spleen lysate; Lane2: Rat Cerebrum lysate; Lane3: Rat Skin lysate; Lane4: HepG2 cell lysate; Lane5: A431 cell lysate Primary Ab: 0.4ug/ml NBP3-41879; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Applications for IL-34 Antibody (C6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5-20ug/mL

Immunohistochemistry

5-20ug/mL

Western Blot

0.5-2ug/mL

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.01M PBS, pH7.4, 50% glycerol

Preservative

0.05% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-34

Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers. It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level.

IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen . The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R). IL-34 stimulates monocyte proliferation. In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes. In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures.

Long Name

Interleukin 34

Alternate Names

C16orf77, IL34

Gene Symbol

IL34

Additional IL-34 Products

Product Documents for IL-34 Antibody (C6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-34 Antibody (C6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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