Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all IL-34 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 13 amino acid synthetic peptide from near the center of human IL-34. The immunogen is located within amino acids 60 - 110 of IL-34. Amino Acid Squence: TRLRAQVSERELR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42-48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-34 Antibody - BSA Free

Western Blot: IL-34 AntibodyBSA Free [NBP1-76356]

Western Blot: IL-34 AntibodyBSA Free [NBP1-76356]

Western Blot: IL-34 Antibody [NBP1-76356] - Analysis of IL-34 in human brain tissue lysate with IL-34 antibody at 0.25 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Western Blot: IL-34 AntibodyBSA Free [NBP1-76356]

Western Blot: IL-34 AntibodyBSA Free [NBP1-76356]

Western Blot: IL-34 Antibody [NBP1-76356] - Analysis of 125 ng of recombinant human IL-34 with IL-34 antibody at 1 ug/ml.

Applications for IL-34 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

0.25 ug/ml
Application Notes
Despite its predicted molecular weight, IL-34 will often run at higher molecular weight in SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-34

Interleukin-34 (IL-34) was identified through a functional screening of a library of secreted proteins after transfection of an embryonic kidney cell line with recombinant cDNAs. Flow cytometry and monocyte viability assays demonstrated increased survival and/or proliferation of monocytes induced by IL-34 and specific binding of IL-34 to CD14+ monocytes. IL-34 is a second ligand to the colony stimulating factor (CSF)-1 receptor in addition to CSF1. Like CSF1, IL-34 stimulates phosphorylation of ERK1 and ERK2 in human monocytes and induces differentiation of bone marrow cells into macrophages. Despite its predicted molecular weight, IL-34 will often run at higher molecular weight in SDS-PAGE.

Long Name

Interleukin 34

Alternate Names

C16orf77, IL34

Gene Symbol

IL34

UniProt

ACD13474 (Human)

Additional IL-34 Products

Product Documents for IL-34 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL-34 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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