IL-36 alpha/IL-1F6 Antibody (OTI1A4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02758

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A4

Concentration

1 mg/ml

View all IL-36 alpha/IL-1F6 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human IL1F6 (NP_055255) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

17.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-36 alpha/IL-1F6 Antibody (OTI1A4)

Western Blot: IL-36 alpha/IL-1F6 Antibody (OTI1A4) [NBP2-02758]

Western Blot: IL-36 alpha/IL-1F6 Antibody (OTI1A4) [NBP2-02758]

Western Blot: IL1F6 Antibody (1A4) [NBP2-02758] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1F6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6.
Immunocytochemistry/Immunofluorescence: IL1F6 Antibody (1A4) [NBP2-02758] Staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1F6.
Flow Cytometry: IL-36 alpha/IL-1F6 Antibody (OTI1A4) [NBP2-02758]

Flow Cytometry: IL-36 alpha/IL-1F6 Antibody (OTI1A4) [NBP2-02758]

Flow Cytometry: IL1F6 Antibody (1A4) [NBP2-02758] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IL1F6 antibody, and then analyzed by flow cytometry.

Applications for IL-36 alpha/IL-1F6 Antibody (OTI1A4)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IL-36 alpha/IL-1F6

Interleukin 36 alpha (IL-36 alpha), also known as IL-1F6 and FIL1 epsilon, is a member of the IL-1 family of proteins. Cells reported to express IL-36 alpha include monocytes, B cells, and T cells. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. The receptor for IL-36 alpha is reported to be a combination of IL-1 R6/IL-1 R rp2 and IL-1 R3/IL-1 R AcP. Recombinant IL-36 alpha has been shown to activate pathways involving NF-kappa B and MAPK in an IL-1 R6/IL-1 R rp2-dependent manner.

Long Name

Interleukin 36 alpha/Interleukin 1 Family 6

Alternate Names

FIL1 epsilon, IL-1E, IL1F6, IL36 alpha, IL36A

Entrez Gene IDs

27179 (Human)

Gene Symbol

IL36A

Additional IL-36 alpha/IL-1F6 Products

Product Documents for IL-36 alpha/IL-1F6 Antibody (OTI1A4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IL-36 alpha/IL-1F6 Antibody (OTI1A4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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