Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all IL-36 beta/IL-1F8 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid peptide near the carboxy terminus of human IL-36B. The immunogen is located within the last 50 amino acids of IL-36B. Amino Acid Squence: QHHHLRKKDKDFSSMR

Specificity

IL-36B antibody is human specific. At least two isoforms of IL-36B are known to exist; this antibody will only detect the longer isoform. IL-36B antibody will not cross-react with IL-36A or IL-36G.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-36 beta/IL-1F8 Antibody - BSA Free

Western Blot: IL-36 beta/IL-1F8 AntibodyBSA Free [NBP2-41239]

Western Blot: IL-36 beta/IL-1F8 AntibodyBSA Free [NBP2-41239]

Western Blot: IL-36 beta/IL-1F8 Antibody [NBP2-41239] - Western blot analysis of IL-36B in A549 cell lysate with IL-36B antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: IL-36 beta/IL-1F8 Antibody - BSA Free [NBP2-41239]

Immunocytochemistry/ Immunofluorescence: IL-36 beta/IL-1F8 Antibody - BSA Free [NBP2-41239]

Immunocytochemistry/Immunofluorescence: IL-36 beta/IL-1F8 Antibody [NBP2-41239] - Immunofluorescence of IL-36B in human lung tissue with IL-36B antibody at 20 ug/mL.
Immunohistochemistry: IL-36 beta/IL-1F8 Antibody - BSA Free [NBP2-41239]

Immunohistochemistry: IL-36 beta/IL-1F8 Antibody - BSA Free [NBP2-41239]

Immunohistochemistry: IL-36 beta/IL-1F8 Antibody [NBP2-41239] - Immunohistochemistry of IL-36B in human lung tissue with IL-36B antibody at 2.5 ug/mL.
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Applications for IL-36 beta/IL-1F8 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/mL

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1 - 2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-36 beta/IL-1F8

Interleukin 36 beta (IL-36 beta), also known as IL-1F8, FIL-1h and IL-1H2, is a member of the IL-1 family of proteins. IL-36 beta is reported to be actively secreted. Cells expressing IL-36 beta include resting and activated monocytes, and B cells. The receptor for IL-36 beta is thought to be a combination of IL-1 Rrp2 and IL-1 RAcP. Recombinant IL-36 beta activates pathways involving NF-kB and MAPK in an IL-1 Rrp2-dependent manner.

Long Name

Interleukin 36 beta/Interleukin 1 Family 8

Alternate Names

FIL1 eta, IL-1 eta, IL-1H2, IL1F8, IL36 beta, IL36B

Gene Symbol

IL36B

Additional IL-36 beta/IL-1F8 Products

Product Documents for IL-36 beta/IL-1F8 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL-36 beta/IL-1F8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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