IL-3R alpha/CD123 Antibody (18N6B12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27225

Novus Biologicals, part of Bio-Techne
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NBP2-27225
NBP2-27225SS
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 18N6B12

Concentration

0.5 mg/ml

View all IL-3R alpha Antibodies »

Product Specifications

Immunogen

A portion of amino acids 250-300 of human CD123 was used as the immunogen for this antibody.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for IL-3R alpha/CD123 Antibody (18N6B12)

Western Blot: IL-3R alpha/CD123 Antibody (18N6B12) [NBP2-27225]

Western Blot: IL-3R alpha/CD123 Antibody (18N6B12) [NBP2-27225]

Western Blot: IL-3 R alpha/CD123 Antibody (18N6B12) [NBP2-27225] - Analysis of CD123 in the A) presence and B) absence of equal amounts antibody and immunizing peptide in human SKNSH cell lysate using CD123 antibody at 10 ug/ml.
Flow Cytometry: IL-3R alpha/CD123 Antibody (18N6B12) [NBP2-27225]

Flow Cytometry: IL-3R alpha/CD123 Antibody (18N6B12) [NBP2-27225]

Flow Cytometry: IL-3 R alpha/CD123 Antibody (18N6B12) [NBP2-27225] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-27225. Staining of CD123 in human buffy coat using anti-CD123 antibody. Image from verified customer review.
Flow Cytometry: IL-3R alpha/CD123 Antibody (18N6B12) [NBP2-27225]

Flow Cytometry: IL-3R alpha/CD123 Antibody (18N6B12) [NBP2-27225]

Flow Cytometry: IL-3 R alpha/CD123 Antibody (18N6B12) [NBP2-27225] - Cell surface analysis using CD123 antibody. BDCM cells were probed using 1 ug of CD123 antibody (red) or 1 ug of isotype control (green). This IL-3 R alpha/CD123 antibody and a mouse IgG-PE secondary antibody were used for this test.
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Applications for IL-3R alpha/CD123 Antibody (18N6B12)

Application
Recommended Usage

Flow Cytometry

1ug/0.5x10^6cells

Western Blot

10ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-3R alpha

CD123 (Cluster of Differentiation 123), also known as Interleukin-3 receptor subunit alpha, is an Interleukin 3 specific subunit of a heterodimeric cytokine receptor. CD123 is a low affinity receptor to interleukin-3 and is composed of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3, colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5. The CD123 beta subunit is activated by the ligand binding, and is required for the biological activities of IL-3. A member of type I cytokine receptor family and type 5 subfamily, CD123 functions as a signaling cytokine known to stimulate cell proliferation, differentiation and survival. The gene for this protein maps within the X-Y pseudoautosomal region (PAR).

Long Name

Interleukin 3 Receptor alpha

Alternate Names

CD123, IL-3 R alpha, IL-3Ra, IL3R alpha, IL3RA

Gene Symbol

IL3RA

UniProt

P26951

Additional IL-3R alpha Products

Product Documents for IL-3R alpha/CD123 Antibody (18N6B12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IL-3R alpha/CD123 Antibody (18N6B12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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