IL-4 Antibody (11B11) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52656

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, In vivo assay

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 11B11

Format

Azide and BSA Free

Concentration

1 mg/ml

View all IL-4 Antibodies »

Product Specifications

Immunogen

BSF-1 / IL-4 purified from serum-free supernatants of cultured EL-4 cells (a C57BL/6 thymoma) induced with 4 Beta-phorbol 12 Beta-myristate 12 alpha -acetate for 48h.

Specificity

Binds to a core functional region of murine IL-4, which prevents this cytokine from interacting with its cognate receptor (Yao et al., 2006 PMID: 16361318).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Applications for IL-4 Antibody (11B11) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry

1:10 - 1:500
Application Notes
This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: IL-4

IL-4 (Interleukin 4, B cell stimulatory factor) is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. IL-4 has many different functions including promoting proliferation and differentiation of immunologically competent cells. One of its main functions is regulating differentiation of naive CD4+ T cells into helper Th2 cells, the other main function is regulating B cells IgE and IgG1 production. Human IL-4 shows 50% homology with mouse IL-4, but its activities are species-specific and human IL-4 shows no activity on murine and rat cells.

Long Name

Interleukin 4

Alternate Names

BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12

Gene Symbol

IL4

Additional IL-4 Products

Product Documents for IL-4 Antibody (11B11) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-4 Antibody (11B11) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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