Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # BVD4-1D11

Concentration

1.0 mg/ml

View all IL-4 Antibodies »

Product Specifications

Immunogen

Recombinant Mouse IL-4

Specificity

This is specific for mouse Interleukin-4 (IL-4). Interleukin-4 is a potent lymphoid growth factor, which stimulates the growth and survival of certain B and T cells. Clone BVD4-1D11 is reported to neutralise the effects of mouse IL-4 (3).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for IL-4 Antibody (BVD4-1D11)

Immunocytochemistry/ Immunofluorescence: IL-4 Antibody (BVD4-1D11) - BSA Free [NB100-64798]

Immunocytochemistry/ Immunofluorescence: IL-4 Antibody (BVD4-1D11) - BSA Free [NB100-64798]

Immunocytochemistry/Immunofluorescence: IL-4 Antibody (BVD4-1D11) [NB100-64798] - Analysis of expression of IL-4 and IL-4 type II receptor subunits in undifferentiated mouse ESCs and mouse primary myoblasts. Representative images of immunocytochemistry analysis of IL-4Ralpha, IL-13Ralpha1, and IL-4 in mouse primary myoblasts at day 6 (white arrows indicate myoblasts, grey arrows indicate myotubes). Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/20/16/3932), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: IL-4 Antibody (BVD4-1D11) - BSA Free [NB100-64798]

Immunocytochemistry/ Immunofluorescence: IL-4 Antibody (BVD4-1D11) - BSA Free [NB100-64798]

Immunocytochemistry/Immunofluorescence: IL-4 Antibody (BVD4-1D11) [NB100-64798] - Analysis of expression of IL-4 and IL-4 type II receptor subunits in undifferentiated mouse ESCs and mouse primary myoblasts. Representative images of immunocytochemistry analysis of IL-4Ralpha, IL-13Ralpha1, and IL-4 in undifferentiated ESCs. Scale bar: 20 um. Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (mdpi.com/1422-0067/20/16/3932), licensed under a CC-BY license.
Western Blot: IL-4 Antibody (BVD4-1D11)BSA Free [NB100-64798]

Western Blot: IL-4 Antibody (BVD4-1D11)BSA Free [NB100-64798]

Western Blot: IL-4 Antibody (BVD4-1D11) [NB100-64798] - Analysis of mouse IL-4 recombinant protein probed with RAT ANTI MOUSE INTERLEUKIN-4 followed by F(ab')2 RABBIT ANTI RAT IgG:HRP.

Applications for IL-4 Antibody (BVD4-1D11)

Application
Recommended Usage

ELISA

1 ug/ml-5 ug/ml

Flow Cytometry

1:10-1:50

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1 ug/ml-5 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-4

IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of native CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Long Name

Interleukin 4

Alternate Names

BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12

Entrez Gene IDs

16189 (Mouse); 16189 (Mouse); 287287 (Rat); 287287 (Rat)

Gene Symbol

IL4

UniProt

P07750

Additional IL-4 Products

Product Documents for IL-4 Antibody (BVD4-1D11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL-4 Antibody (BVD4-1D11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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