IL-4R alpha Antibody (25463 (49/20))

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65838

Novus Biologicals, part of Bio-Techne
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NB100-65838
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 25463 (49/20)

Concentration

1.0 mg/ml

View all IL-4R alpha Antibodies »

Product Specifications

Immunogen

Spodoptera frugiperda lepidopteran ovarian cell line Sf21-derived, recombinant human interleukin 4 receptor, extracellular domain

Specificity

NB100-65838 is specific for human interleukin 4 receptor, the transmembrane ligand-binding subunit of the heterodimeric IL-4 receptor complex. IL-4 R is expressed by B cells, T cells, monocytes and macrophages. NB100-65838 blocks human cell surface IL-4 receptor mediated bioactivities induced by IL-4 or IL-13. An ND50 was observed at approximately 5-20 ng/ml in the presence of 0.2 ng/ml of IL-4 using the factor dependant human erythroleukemic cell line, TF-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for IL-4R alpha Antibody (25463 (49/20))

Application
Recommended Usage

ELISA

1:500-1:2000

Flow Cytometry

1:20-1:80

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:40-1:125

Western Blot

1:250-1:1000
Application Notes
Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry and Functional In WB will detect human IL-4 R under non-reducing conditions.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.5% Trehalose

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IL-4R alpha

IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Long Name

Interleukin 4 Receptor alpha

Alternate Names

CD124, IL-4 R alpha, IL-4Ra, IL4R, IL4R alpha

Entrez Gene IDs

3566 (Human)

Gene Symbol

IL4R

UniProt

P24394 (Human)

Additional IL-4R alpha Products

Product Documents for IL-4R alpha Antibody (25463 (49/20))

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IL-4R alpha Antibody (25463 (49/20))

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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