IL-4R alpha Antibody (dupilumab)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-28009

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG4 Clone # dupilumab

Concentration

LYOPH mg/ml

View all IL-4R alpha Antibodies »

Product Specifications

Immunogen

IL-4Ra / CD124

Reactivity Notes

Predicted species reactivity: Mouse, Cynomolgus

Clonality

Monoclonal

Host

Human

Isotype

IgG4

Endotoxin Level

< 0.001EU/ug,determined by LAL method.

Description

Expressed from CHO. The heavy chain type is huIgG4SP, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Also known as 'dupilumab'.

Upon receipt, store immediately at -20C or lower for 24 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Scientific Data Images for IL-4R alpha Antibody (dupilumab)

IL-4R alpha Antibody (dupilumab)

ELISA: IL-4R alpha Antibody (dupilumab)[NBP3-28009] -

Immobilized human IL4RA His at 2 ug/mL can bind IL-4R alpha Antibody (dupilumab), EC50=0.004327 ug/mL.
IL-4R alpha Antibody (dupilumab)

Flow Cytometry: IL-4R alpha Antibody (dupilumab)[NBP3-28009] -

Human IL-4Ra / CD124 HEK293 Cell Line were stained with dupilumab and negative control protein respectively, washed and then followed by FITC and analyzed with FACS,EC50=0.227 nM.

Applications for IL-4R alpha Antibody (dupilumab)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: IL-4R alpha

IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of na ve CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Long Name

Interleukin 4 Receptor alpha

Alternate Names

CD124, IL-4 R alpha, IL-4Ra, IL4R, IL4R alpha

Gene Symbol

IL4R

UniProt

P24394 (Human)

Additional IL-4R alpha Products

Product Documents for IL-4R alpha Antibody (dupilumab)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL-4R alpha Antibody (dupilumab)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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