IL-6 Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42762
Key Product Details
Species Reactivity
Human, Mouse, Rat, Monkey
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Label
HRP
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli. (Uniprot: P05231)
Reactivity Notes
Use in Rat reported in scientific literature (PMID:33651836).
Localization
Secreted
Specificity
Anti-IL-6 antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for IL-6 Antibody [HRP]
Western Blot: IL-6 Antibody [HRP] [NBP1-42762] - Lane 1: Human IL-6. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase Human IL-6 secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa for Human IL-6. Other band(s): none.
![IL-6 Antibody [HRP]](https://resources.bio-techne.com/images/products/nbp1-42762_rabbit-polyclonal-il-6-antibody-hrp-25520231255152.jpg "IL-6 Antibody [HRP]")
IL-6 Antibody [HRP]
Western Blot of Rabbit Anti-IL-6 Antibody Peroxidase Conjugated. Lane 1: Opal Prestained Molecular Weight MarkerApplications for IL-6 Antibody [HRP]
Application
Recommended Usage
ELISA
1:10000-1:50000
Immunohistochemistry
1:500-1:2500
Immunohistochemistry-Paraffin
1:500-1:2500
Western Blot
1:1000-1:5000
Application Notes
This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IL-6
Long Name
Interleukin 6
Alternate Names
BSF-2, BSF2, IFNB2, IL6, MGI-2A
Gene Symbol
IL6
UniProt
Additional IL-6 Products
Product Documents for IL-6 Antibody [HRP]
Product Specific Notices for IL-6 Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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