IL-6R alpha Antibody (033)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90512

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Sandwich ELISA Capture

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 033

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IL-6R alpha Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse IL-6R alpha (Accession#: NP_034689.2; Ile44-Glu357).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for IL-6R alpha Antibody (033)

Immunocytochemistry/ Immunofluorescence: IL-6R alpha Antibody (033) [NBP2-90512]

Immunocytochemistry/ Immunofluorescence: IL-6R alpha Antibody (033) [NBP2-90512]

Immunocytochemistry/Immunofluorescence: IL-6R alpha Antibody (033) [NBP2-90512] - Immunofluorescence staining of Mouse IL-6R alpha in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse IL-6R alpha monoclonal antibody (1:60) at 37C 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to cytoplasmic and cell membrane.
Flow Cytometry: IL-6R alpha Antibody (033) [NBP2-90512]

Flow Cytometry: IL-6R alpha Antibody (033) [NBP2-90512]

Flow Cytometry: IL-6R alpha Antibody (033) [NBP2-90512] - Flow cytometric analysis of Mouse IL-6R alpha expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse IL-6R alpha, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for IL-6R alpha Antibody (033)

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Sandwich ELISA Capture

1:250-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-6R alpha

IL-6 R alpha is a transmembrane subunit of the receptor for Interleukin-6. It associates with gp130 which is a shared component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. IL-6 plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis.

Long Name

Interleukin 6 Receptor alpha

Alternate Names

CD126, IL-6 R alpha, IL-6Ra, IL6R, IL6R alpha

Gene Symbol

IL6R

Additional IL-6R alpha Products

Product Documents for IL-6R alpha Antibody (033)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IL-6R alpha Antibody (033)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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