IL-7R alpha/CD127 Antibody (22F3D9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22376

Novus Biologicals, part of Bio-Techne
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NBP2-22376
NBP2-22376SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Lambda Clone # 22F3D9

Format

BSA Free

Concentration

1.0 mg/ml

View all IL-7R alpha/CD127 Antibodies »

Product Specifications

Immunogen

Amino acids 141-237 of human CD127 were used as the immunogen for the antibody.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Lambda

Scientific Data Images for IL-7R alpha/CD127 Antibody (22F3D9) - BSA Free

Western Blot: IL-7R alpha/CD127 Antibody (22F3D9)BSA Free [NBP2-22376]

Western Blot: IL-7R alpha/CD127 Antibody (22F3D9)BSA Free [NBP2-22376]

Western Blot: IL-7R alpha/CD127 Antibody (22F3D9) [NBP2-22376] - Analysis of CD127 in A) a partial recombinant protein and B) Daudi lysate using CD127 antibody at 0.5 ug/ml and 5 ug/ml respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Flow Cytometry: IL-7R alpha/CD127 Antibody (22F3D9) - BSA Free [NBP2-22376]

Flow Cytometry: IL-7R alpha/CD127 Antibody (22F3D9) - BSA Free [NBP2-22376]

Flow Cytometry: IL-7R alpha/CD127 Antibody (22F3D9) [NBP2-22376] - Analysis of IL-7 R alpha in human PMBCs using anti-IL-7 R alpha antibody. Image from verified customer review. Image using the Alexa Fluor 405 format of this antibody.

Applications for IL-7R alpha/CD127 Antibody (22F3D9) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:100. Use reported by customer review

Western Blot

2-5 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. The amino acid sequence used as immunogen for the antibody is present in two isoforms of CD127: 459 amino acids and 299 amino acids. This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.1 ml PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-7R alpha/CD127

Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. The human and mouse sequence is nearly identical. In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged.

Long Name

Interleukin 7 Receptor alpha

Alternate Names

CD127, IL-7 R alpha, IL-7Ra, IL7R, IL7R alpha

Gene Symbol

IL7R

UniProt

NP_002176.2 (Human)

Additional IL-7R alpha/CD127 Products

Product Documents for IL-7R alpha/CD127 Antibody (22F3D9) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IL-7R alpha/CD127 Antibody (22F3D9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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