IL-7R alpha/CD127 [p Tyr449] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-85537

Novus Biologicals, part of Bio-Techne
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NB110-85537
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IL-7R alpha/CD127 Antibodies »

Product Specifications

Immunogen

This affinity purified IL-7R alpha/CD127 [p Tyr449] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near Y449 of mouse IL-7R alpha/CD127R alpha/CD127 protein. (Uniprot: P16872)

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with IL-7R alpha/CD127 from human, mouse and rat sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-7R alpha/CD127 from other sources has not been determined.

Modification

p Tyr449

Specificity

This antibody is specific for mouse IL-7R alpha/CD127 protein phosphorylated at Y449.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against non-phospho-peptide

Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for IL-7R alpha/CD127 [p Tyr449] Antibody

Western Blot: IL-7R alpha/CD127 [p Tyr449] Antibody [NB110-85537]

Western Blot: IL-7R alpha/CD127 [p Tyr449] Antibody [NB110-85537]

Western Blot: IL-7 R alpha/CD127 [p Tyr449] Antibody [NB110-85537] - Lane 1: thymocyte D1 cells. Lane 2: thymocyte D1 cells treated with IL7 (50 ng/ml). IP: with anti-Phosphotyrosine conjugated to Protein G agarose. Primary antibody: IL7pY449 antibody at 1:10000 for overnight at 4C. Secondary antibody: HRP-conjugated goat anti-rabbit antibody at 1:10,000 for 45 min at RT and ECL detection. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa, 64 kDa for IL7pY449. Other band(s): unspecifics.
Western Blot: IL-7R alpha/CD127 [p Tyr449] Antibody [NB110-85537]

Western Blot: IL-7R alpha/CD127 [p Tyr449] Antibody [NB110-85537]

Western Blot: IL-7 R alpha/CD127 [p Tyr449] Antibody [NB110-85537] - Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa, 64 kDa for IL7pY449. Other band(s): unspecifics.
IL-7R alpha/CD127 [p Tyr449] Antibody

IL-7R alpha/CD127 [p Tyr449] Antibody

Western Blot and immunoprecipitation of Rabbit aeceptor-alpha-chain-pY449 antibody. Lane 1: thymocyte D1 cells. Lane 2: thymocyte D1 cells treated with IL7 (50 ng/ml). IP: with anti-Phosphotyrosine conjugated to Protein G agarose. Primary antibody: IL7pY449 antibody at 1:10000 for overnight at 4C. Secondary antibody: HRP-conjugated goat anti-rabbit antibody at 1:10,000 for 45 min at RT and ECL detection. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.
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Applications for IL-7R alpha/CD127 [p Tyr449] Antibody

Application
Recommended Usage

ELISA

1:5000-1:50000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:10000
Application Notes
This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 55 kDa in size corresponding to phosphorylated IL-7 by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse IL-7 pY449 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IL-7R alpha/CD127

Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. The human and mouse sequence is nearly identical. In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged.

Long Name

Interleukin 7 Receptor alpha

Alternate Names

CD127, IL-7 R alpha, IL-7Ra, IL7R, IL7R alpha

Entrez Gene IDs

3575 (Human); 16197 (Mouse); 294797 (Rat)

Gene Symbol

IL7R

UniProt

P16872

Additional IL-7R alpha/CD127 Products

Product Documents for IL-7R alpha/CD127 [p Tyr449] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IL-7R alpha/CD127 [p Tyr449] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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