IL-9R Antibody (AH9R2) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12033

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-12033-0.025mg
NBP3-12033-0.2mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # AH9R2

Format

Azide and BSA Free

Concentration

1 mg/ml

View all IL-9R Antibodies »

Product Summary for IL-9R Antibody (AH9R2) - Chimeric - Azide and BSA Free

Immunogen

This antibody was raised by immunising DBA/2 mice with P815 mastocytoma cells transfected with human IL-9R cDNA.

Specificity

This antibody binds specifically to the human IL-9 receptor alpha chain.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for IL-9R Antibody (AH9R2) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: IL-9R Antibody (AH9R2) - Chimeric - Azide and BSA Free [NBP3-12033]

Immunocytochemistry/ Immunofluorescence: IL-9R Antibody (AH9R2) - Chimeric - Azide and BSA Free [NBP3-12033]

Immunocytochemistry/Immunofluorescence: IL-9R Antibody (AH9R2) - Chimeric [NBP3-12033] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of AH9R2 (NBP3-12033) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12033, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.
Flow Cytometry: IL-9R Antibody (AH9R2) - Chimeric - Azide and BSA Free [NBP3-12033]

Flow Cytometry: IL-9R Antibody (AH9R2) - Chimeric - Azide and BSA Free [NBP3-12033]

Flow Cytometry: IL-9R Antibody (AH9R2) - Chimeric [NBP3-12033] - Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of HB-8592 (NBP3-12033, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Applications for IL-9R Antibody (AH9R2) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: IL-9R

The protein encoded by the IL9R gene is a cytokine receptor that specifically mediates the biological effects ofinterleukin 9 (IL9). The functional IL9 receptor complex requires this protein as well as the interleukin 2 receptor,gamma (IL2RG), a common gamma subunit shared by the receptors of many different cytokines. The ligand binding of thisreceptor leads to the activation of various JAK kinases and STAT proteins, which connect to different biologicresponses. This gene is located at the pseudoautosomal regions of X and Y chromosomes. Genetic studies suggested anassociation of this gene with the development of asthma. Multiple pseudogenes on chromosome 9, 10, 16, and 18 havebeen described. Alternatively spliced transcript variants have been found for this gene. (provided by RefSeq)

Long Name

Interleukin 9 Receptor

Alternate Names

CD129, IL-9 R, IL9R

Gene Symbol

IL9R

Additional IL-9R Products

Product Documents for IL-9R Antibody (AH9R2) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-9R Antibody (AH9R2) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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