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Key Product Details

Species Reactivity

Human, Mouse, Canine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6F1

Concentration

1 mg/ml

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human ILF2 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens interleukin enhancer binding factor 2, 45kDa (ILF2).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ILF2 Antibody (OTI6F1)

Western Blot: ILF2 Antibody (OTI6F1) [NBP1-47811]

Western Blot: ILF2 Antibody (OTI6F1) [NBP1-47811]

Western Blot: ILF2 Antibody (OTI6F1) [NBP1-47811] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ILF2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: ILF2 Antibody (OTI6F1) [NBP1-47811]

Immunocytochemistry/ Immunofluorescence: ILF2 Antibody (OTI6F1) [NBP1-47811]

Immunocytochemistry/Immunofluorescence: ILF2 Antibody (OTI6F1) [NBP1-47811] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ILF2.
Immunohistochemistry-Paraffin: ILF2 Antibody (OTI6F1) [NBP1-47811]

Immunohistochemistry-Paraffin: ILF2 Antibody (OTI6F1) [NBP1-47811]

Immunohistochemistry-Paraffin: ILF2 Antibody (OTI6F1) [NBP1-47811] - Staining of paraffin-embedded Kidney tissue using anti-ILF2 mouse monoclonal antibody.

Applications for ILF2 Antibody (OTI6F1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ILF2

Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2.In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of thisgene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression.

Alternate Names

interleukin enhancer binding factor 2, 45kD, interleukin enhancer binding factor 2, 45kDa, interleukin enhancer-binding factor 2, MGC8391, NF45PRO3063, Nuclear factor of activated T-cells 45 kDa, nuclear factor of activated T-cells, 45-kDa

Entrez Gene IDs

3608 (Human)

Gene Symbol

ILF2

Additional ILF2 Products

Product Documents for ILF2 Antibody (OTI6F1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ILF2 Antibody (OTI6F1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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