ILF3 Antibody (0S2N7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16664

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16664-100ul
NBP3-16664-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0S2N7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ILF3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ILF3 (Q12906). MRPMRIFVNDDRHVMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKGSSEQAESDNMDVPPEDDSKEGAGEQKTEHMTRTLRGVMRVGLVAK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ILF3 Antibody (0S2N7)

Western Blot: ILF3 Antibody (0S2N7) [NBP3-16664]

Western Blot: ILF3 Antibody (0S2N7) [NBP3-16664]

Western Blot: ILF3 Antibody (0S2N7) [NBP3-16664] - Western blot analysis of extracts of various cell lines, using ILF3 Rabbit mAb (NBP3-16664) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: ILF3 Antibody (0S2N7) [NBP3-16664]

Immunohistochemistry-Paraffin: ILF3 Antibody (0S2N7) [NBP3-16664]

Immunohistochemistry-Paraffin: ILF3 Antibody (0S2N7) [NBP3-16664] - Immunohistochemistry of paraffin-embedded mouse kidney using ILF3 Rabbit mAb (NBP3-16664) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: ILF3 Antibody (0S2N7) [NBP3-16664]

Immunohistochemistry-Paraffin: ILF3 Antibody (0S2N7) [NBP3-16664]

Immunohistochemistry-Paraffin: ILF3 Antibody (0S2N7) [NBP3-16664] - Immunohistochemistry of paraffin-embedded rat testis using ILF3 Rabbit mAb (NBP3-16664) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for ILF3 Antibody (0S2N7)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ILF3

ILF3 encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein was first discovered to be a subunit of the nuclear factor of activated T-cells (NFAT); a transcription factor required for T-cell expression of interleukin 2. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. These proteins have been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth; possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Alternate Names

Double-stranded RNA-binding protein 76, double-stranded RNA-binding protein, 76 kD, DRBF, DRBP76M-phase phosphoprotein 4, dsRNA binding protein NFAR-2/MPP4, interleukin enhancer binding factor 3, 90kDa, interleukin enhancer-binding factor 3, MPHOSPH4interleukin enhancer binding factor 3, 90kD, MPP4MMP4, NF90CBTF, NFAR-1, NFAR2, NFARNF110, NF-AT-90, Nuclear factor associated with dsRNA, Nuclear factor of activated T-cells 90 kDa, nuclear factor of activated T-cells, 90 kD, TCP110, TCP80, Translational control protein 80

Gene Symbol

ILF3

Additional ILF3 Products

Product Documents for ILF3 Antibody (0S2N7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ILF3 Antibody (0S2N7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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