ILK Antibody (3A9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37448

Novus Biologicals, part of Bio-Techne
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NBP2-37448
NBP2-37448-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Monkey, Primate

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3A9

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all ILK Antibodies »

Product Summary for ILK Antibody (3A9) - BSA Free

Immunogen

Purified recombinant fragment of human ILK (AA: 97-244) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

51.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ILK Antibody (3A9) - BSA Free

Western Blot: ILK Antibody (3A9)BSA Free [NBP2-37448]

Western Blot: ILK Antibody (3A9)BSA Free [NBP2-37448]

Western Blot: ILK Antibody (3A9) [NBP2-37448] - Analysis using ILK mouse mAb against Jurkat (1), NIH3T3 (2), HeLa (3), PC-12 (4), C6 (5), COS7 (6), Raji (7), K562 (8) and MCF-7 (9) cell lysate.
Immunohistochemistry-Paraffin: ILK Antibody (3A9) - BSA Free [NBP2-37448]

Immunohistochemistry-Paraffin: ILK Antibody (3A9) - BSA Free [NBP2-37448]

Immunohistochemistry-Paraffin: ILK Antibody (3A9) [NBP2-37448] - Analysis of rectum cancer tissues using ILK mouse mAb with DAB staining.
Flow Cytometry: ILK Antibody (3A9) - BSA Free [NBP2-37448]

Flow Cytometry: ILK Antibody (3A9) - BSA Free [NBP2-37448]

Flow Cytometry: ILK Antibody (3A9) [NBP2-37448] - Analysis of Jurkat cells using ILK mouse mAb (green) and negative control (red).
View 3 more images

Applications for ILK Antibody (3A9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ILK

Integrin-linked kinase (ILK) is a key cytoplasmic plaque protein involved in the mediation of integrin functions. It plays a key role as a regulator during cell survival, cell proliferation, cell migration, and cell to cell adhesion (1). ILK links integrins to the actin cytoskeleton, allowing transduction of signals through integrins to the extracellular matrix (ECM). Also, ILK has been linked to the regulation of smooth contraction and cell motility in non-muscle cells through phosphorylation of myosin (2). Irregular activity level of ILK has been linked to expression of certain tumor. ILK overexpression caused by inactivating mutation in tumor suppressors (e.g. PTEN and APC) lead to tumor formation in transgenic mouse. Up-regulated ILK expression and activity are seen in Ewings scaromas, colorectal cancers, and late staged prostate cancers (3).

Long Name

Integrin-linked Kinase

Alternate Names

ESTM24, p59

Gene Symbol

ILK

Additional ILK Products

Product Documents for ILK Antibody (3A9) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ILK Antibody (3A9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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