ILK Antibody (6A5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26198
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6A5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ILK Antibody (6A5)
Immunogen
A synthesized peptide derived from Human ILK [UniProt Q13418]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ILK Antibody (6A5)
Immunoprecipitation: ILK Antibody (6A5) [NBP3-26198] -
Immunoprecipitation: ILK Antibody (6A5) [NBP3-26198] - Immunoprecipitating ILK in Hela whole cell lysate.Lane 1: Rabbit control IgG instead of NBP3-26198 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26198 (2ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (10ug).
Western Blot: ILK Antibody (6A5) [NBP3-26198] -
Western Blot: ILK Antibody (6A5) [NBP3-26198] - Positive Western Blot detected in: Hela whole cell lysate, HL60 whole cell lysate, A549 whole cell lysate, Mouse kidney tissueAll lanes: ILK Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 52, 45, 37 kDa
Observed band size: 51 kDa
Immunocytochemistry/Immunofluorescence: ILK Antibody (6A5) [NBP3-26198] -
Immunocytochemistry/Immunofluorescence: ILK Antibody (6A5) [NBP3-26198] - Staining of MCF7 Cells with ILK Antibody (6A5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).Applications for ILK Antibody (6A5)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunoprecipitation
1:200-1:1000
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: ILK
Long Name
Integrin-linked Kinase
Alternate Names
ESTM24, p59
Gene Symbol
ILK
Additional ILK Products
Product Documents for ILK Antibody (6A5)
Product Specific Notices for ILK Antibody (6A5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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