Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2H3

Concentration

1 mg/ml

View all ILK Antibodies »

Product Summary for ILK Antibody (OTI2H3)

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-244 of human ILK (NP_004508) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

51.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ILK Antibody (OTI2H3)

Western Blot: ILK Antibody (OTI2H3) [NBP2-45415]

Western Blot: ILK Antibody (OTI2H3) [NBP2-45415]

Western Blot: ILK Antibody (2H3) [NBP2-45415] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ILK.

Applications for ILK Antibody (OTI2H3)

Application
Recommended Usage

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ILK

Integrin-linked kinase (ILK) is a key cytoplasmic plaque protein involved in the mediation of integrin functions. It plays a key role as a regulator during cell survival, cell proliferation, cell migration, and cell to cell adhesion (1). ILK links integrins to the actin cytoskeleton, allowing transduction of signals through integrins to the extracellular matrix (ECM). Also, ILK has been linked to the regulation of smooth contraction and cell motility in non-muscle cells through phosphorylation of myosin (2). Irregular activity level of ILK has been linked to expression of certain tumor. ILK overexpression caused by inactivating mutation in tumor suppressors (e.g. PTEN and APC) lead to tumor formation in transgenic mouse. Up-regulated ILK expression and activity are seen in Ewings scaromas, colorectal cancers, and late staged prostate cancers (3).

Long Name

Integrin-linked Kinase

Alternate Names

ESTM24, p59

Gene Symbol

ILK

Additional ILK Products

Product Documents for ILK Antibody (OTI2H3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ILK Antibody (OTI2H3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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