ILK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-60451

Novus Biologicals, part of Bio-Techne
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NB100-60451
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Guinea Pig (100%), Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all ILK Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 402 and 451 of human integrin-linked kinase using the numbering given in entry NP_004508.1 (GeneID 3611).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ILK Antibody

Western Blot: ILK Antibody [NB100-60451]

Western Blot: ILK Antibody [NB100-60451]

Western Blot: ILK Antibody [NB100-60451] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. NB100-60451 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.
Immunocytochemistry/ Immunofluorescence: ILK Antibody [NB100-60451]

Immunocytochemistry/ Immunofluorescence: ILK Antibody [NB100-60451]

Immunocytochemistry/Immunofluorescence: ILK Antibody [NB100-60451] - Acetone-fixed asynchronous HeLa cells. Antibody used at a dilution of 1:100 (2ug/ml).

Applications for ILK Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Acetone fixation is recommended. Permeabilization is not recommended. Formaldehyde fixation is not recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ILK

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Long Name

Integrin-linked Kinase

Alternate Names

ESTM24, p59

Entrez Gene IDs

3611 (Human); 16202 (Mouse)

Gene Symbol

ILK

UniProt

Q13418

Additional ILK Products

Product Documents for ILK Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ILK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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