ILK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-809

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB100-809
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ILK Antibodies »

Product Summary for ILK Antibody

Immunogen

Peptide with sequence C-DMIVPILEKMQDK corresponding to C-Terminus according to NP_004508.1, NP_001014794.1, NP_001014795.1.

Specificity

All 3 varients (represented by NP_004508.1; NP_001014794.1 and NP_001014795.1) encode the same protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ILK Antibody

Western Blot: ILK Antibody [NB100-809]

Western Blot: ILK Antibody [NB100-809]

Western Blot: ILK Antibody [NB100-809] - (0.01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ILK Antibody [NB100-809]

Immunohistochemistry-Paraffin: ILK Antibody [NB100-809]

Immunohistochemistry-Paraffin: ILK Antibody [NB100-809] - (4ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Western Blot: ILK Antibody [NB100-809]

Western Blot: ILK Antibody [NB100-809]

Western Blot: ILK Antibody [NB100-809] - (0.03ug/ml) staining of Pig (A), Mouse (B) and Rat (C) Liver lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for ILK Antibody

Application
Recommended Usage

Immunohistochemistry

2-4ug/ml

Immunohistochemistry-Paraffin

2-4ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.01-0.03ug/ml
Application Notes
Western blot: Approx. 37kDa band observed in Human, Mouse, Rat and Pig Liver lysate (calculated MW of 34.7kDa according to NP_000036.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.
IHC: In paraffin embedded Human Kidney shows preferential staining of PCT.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ILK

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Long Name

Integrin-linked Kinase

Alternate Names

ESTM24, p59

Entrez Gene IDs

3611 (Human); 11846 (Mouse); 29221 (Rat)

Gene Symbol

ILK

UniProt

NP_004508.1 (Human)

Additional ILK Products

Product Documents for ILK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ILK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.