ILT5/CD85a/LILRB3 Antibody (058)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90707

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-90707-100ul
NBP2-90707-50ul
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 058

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all LILRB3/CD85a/ILT5 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse ILT5/CD85a/LILRB3 (Accession#: AAC53219.1; Met1-Tyr640).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ILT5/CD85a/LILRB3 Antibody (058)

Immunocytochemistry/ Immunofluorescence: ILT5/CD85a/LILRB3 Antibody (058) [NBP2-90707]

Immunocytochemistry/ Immunofluorescence: ILT5/CD85a/LILRB3 Antibody (058) [NBP2-90707]

Immunocytochemistry/Immunofluorescence: ILT5/CD85a/LILRB3 Antibody (058) [NBP2-90707] - Immunofluorescence staining of mouse LILRB3 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse LILRB3 monoclonal antibody (1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
Flow Cytometry: ILT5/CD85a/LILRB3 Antibody (058) [NBP2-90707]

Flow Cytometry: ILT5/CD85a/LILRB3 Antibody (058) [NBP2-90707]

Flow Cytometry: ILT5/CD85a/LILRB3 Antibody (058) [NBP2-90707] - Flow cytometric analysis of Mouse LILRB3(CD85a) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse LILRB3(CD85a), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for ILT5/CD85a/LILRB3 Antibody (058)

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LILRB3/CD85a/ILT5

LILRB3 is a 631 amino acid type I transmembrane glycoprotein, which contains four immunoreceptor tyrosine-based inhibition motif (ITIM) sequences within a long cytoplasmic tail. Phosphorylation of the tyrosine residues within ITIMs is known to enable the binding and activation of protein tyrosine phosphatases, which act as cell signaling modulators and inhibitors of cell activation. LILRB3 may act as receptor for class I MHC antigens.

Alternate Names

CD85a, ILT5, LIR3

Gene Symbol

LILRB3

Additional LILRB3/CD85a/ILT5 Products

Product Documents for ILT5/CD85a/LILRB3 Antibody (058)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ILT5/CD85a/LILRB3 Antibody (058)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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