IMP2/IGF2BP2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-93408
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Canine (100%), Mouse (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-KQQEQKYPQGVASQR corresponding to C-Terminus according to NP_006539.3, NP_001007226.1.
Specificity
This antibody is expected to recognise both reported isoforms (NP_006539.3 and NP_001007226.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IMP2/IGF2BP2 Antibody
Western Blot: IMP2/IGF2BP2 Antibody [NB100-93408]
Western Blot: IGF2BP2 Antibody [NB100-93408] - HEK293 overexpressing IGF2BP2 and probed with (mock transfection in first lane).Western Blot: IMP2/IGF2BP2 Antibody [NB100-93408]
Western Blot: IGF2BP2 Antibody [NB100-93408] - (1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Applications for IMP2/IGF2BP2 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:16000
Western Blot
1 - 3 ug/ml
Application Notes
WB: Approx. 60-70 kDa band observed in HepG2 lysates (calculated MW of 66.1 kDa band according to NP_006539.3). In transfected HEK293 transiently expressing IGF2BP2 a band of approx. 65 kDa is observed. This band is not observed in the non-transfected HEK293.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IMP2
Long Name
Insulin-like Growth Factor 2 mRNA Binding Protein 2
Alternate Names
IF2B2, IGF2BP2, VICKZ2
Gene Symbol
IGF2BP2
UniProt
Additional IMP2 Products
Product Documents for IMP2/IGF2BP2 Antibody
Product Specific Notices for IMP2/IGF2BP2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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