Importin alpha 2/KPNA2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1371

Novus Biologicals, part of Bio-Techne
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NB100-1371
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Importin alpha 2/KPNA2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QVQDGAPGTFNF corresponding to C-Terminus according to NP_002257.1.

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Canine

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Importin alpha 2/KPNA2 Antibody

Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-1371]

Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-1371]

Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-1371] - Staining of Hela lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 0.03 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NB100-1371]

Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NB100-1371]

Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NB100-1371] - Staining of paraffin embedded Human Testis. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Importin alpha 2/KPNA2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.03 - 0.1 ug/mL
Application Notes
WB: Approx 55 kDa band observed in lysates of cell line HeLa (calculated MW of 57.9 kDa according to NP_002257.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Importin alpha 2/KPNA2

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination.

Long Name

Karyopherin alpha 2

Alternate Names

Importin alpha 1, IPOA1, KPNA2, Pendulin, QIP2, RCH1, SRP1-alpha

Entrez Gene IDs

3838 (Human); 16647 (Mouse); 85245 (Rat)

Gene Symbol

KPNA2

UniProt

NP_002257. (Human)

Additional Importin alpha 2/KPNA2 Products

Product Documents for Importin alpha 2/KPNA2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Importin alpha 2/KPNA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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