Importin alpha 3/KPNA4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-81651

Novus Biologicals, part of Bio-Techne
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NB100-81651
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Importin alpha 3/KPNA4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 471 and 521 of human karyopherin alpha 4 using the numbering given in entry NP_002259.1 (GeneID 3840).

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32320654).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Importin alpha 3/KPNA4 Antibody

Western Blot: Importin alpha 3/KPNA4 Antibody [NB100-81651]

Western Blot: Importin alpha 3/KPNA4 Antibody [NB100-81651]

Western Blot: Importin alpha 3/KPNA4 Antibody [NB100-81651] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-81651 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate.
Immunohistochemistry-Paraffin: Importin alpha 3/KPNA4 Antibody [NB100-81651]

Immunohistochemistry-Paraffin: Importin alpha 3/KPNA4 Antibody [NB100-81651]

Immunohistochemistry-Paraffin: Importin alpha 3/KPNA4 Antibody [NB100-81651] - Human prostate carcinoma. Antibody used at a dilution of 1:200.

Applications for Importin alpha 3/KPNA4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Importin alpha 3/KPNA4

The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.

Long Name

Karyopherin alpha 4

Alternate Names

KPNA4, QIP12, SRP3

Entrez Gene IDs

3840 (Human)

Gene Symbol

KPNA4

UniProt

NP_002259.1 (Human)

Additional Importin alpha 3/KPNA4 Products

Product Documents for Importin alpha 3/KPNA4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Importin alpha 3/KPNA4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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