ING1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-908
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity purified ING1 Antibody was prepared from whole Goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 275-300 of Human ING1 protein (Inhibitor of growth family, member 1). (Uniprot: Q9UK53)
Reactivity Notes
Reactivity occurs against human p33 ING11 protein and is 100% homologous to all splice variants for ING11. Cross reactivity with p33 ING11 protein from mouse and rat sources is expected based on high homology (one amino acid change in sequence) by BLAST. Reactivity with p33 ING11 proteins from other sources is not known.
Localization
Nuclear
Specificity
Reactivity occurs against human p33 ING11 protein and is 100% homologous to all splice variants for ING11. Cross reactivity with p33 ING11 protein from mouse and rat sources is expected based on high homology (one amino acid change in sequence) by BLAST. Reactivity with p33 ING11 proteins from other sources is not known.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for ING1 Antibody
![Western Blot: ING1 Antibody [NB600-908]](https://resources.bio-techne.com/images/products/ING1-Antibody-Western-Blot-NB600-908-img0006.jpg "Western Blot: ING1 Antibody [NB600-908]")
Western Blot: ING1 Antibody [NB600-908]
Western Blot: ING1 Antibody [NB600-908] - Western blot analysis is shown using Affinity Purified ING1 antibody to detect over expressed Human ING1 present in cell nuclear extracts. This western blot shows reactivity with purified recombinant GST tagged human ING1b (lane 1, ~33kDa) and ING1c protein (lane 2, ~24kDa). No reactivity is seen against GST or other ING proteins (not shown). Reactivity of this polyclonal antibody is similar to the specificity of a positive control monoclonal antibody (not shown). The blot was incubated with a 1:1,000 dilution of the antibody at room temperature followed by detection using standard techniques.
N/A: ING1 Antibody [NB600-908]
Applications for ING1 Antibody
Application
Recommended Usage
ELISA
1:4000-1:14000
Western Blot
1:500-1:3000
Application Notes
This affinity-purified antibody has been tested for use in ELISA and western blotting using over expressed cell lysates. Expect a band approximately 47 kDa, 33 kDa or 24 kDa in size corresponding to variants of ING1 by western blotting in the appropriate cell lysate or extract. Specific conditions for other applications should be optimized by the end user.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: ING1
Long Name
Inhibitor of Growth 1
Alternate Names
p24ING1c, p33ING1b, p47ING1a
Entrez Gene IDs
3621 (Human)
Gene Symbol
ING1
UniProt
Additional ING1 Products
Product Documents for ING1 Antibody
Product Specific Notices for ING1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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