iNOS Antibody (3L0U6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16273

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16273-100ul
NBP3-16273-20ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3L0U6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all iNOS Antibodies »

Product Summary for iNOS Antibody (3L0U6)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 2-200 of human iNOS (P35228). ACPWKFLFKTKFHQYAMNGEKDINNNVEKAPCATSSPVTQDDLQYHNLSKQQNESPQPLVETGKKSPESLVKLDATPLSSPRHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGSIMTPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRC

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for iNOS Antibody (3L0U6)

Western Blot: iNOS Antibody (3L0U6) [NBP3-16273]

Western Blot: iNOS Antibody (3L0U6) [NBP3-16273]

Western Blot: iNOS Antibody (3L0U6) [NBP3-16273] - Western blot analysis of extracts of RAW264.7 cells, using iNOS antibody (NBP3-16273) at 1:1000 dilution.RAW264.7 cells were treated by LPS (1 ug/ml) at 37C for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunocytochemistry/ Immunofluorescence: iNOS Antibody (3L0U6) [NBP3-16273]

Immunocytochemistry/ Immunofluorescence: iNOS Antibody (3L0U6) [NBP3-16273]

Immunocytochemistry/Immunofluorescence: iNOS Antibody (3L0U6) [NBP3-16273] - Immunofluorescence analysis of Raw264 7 cells using iNOS Rabbit mAb (NBP3-16273) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: iNOS Antibody (3L0U6) [NBP3-16273]

Immunocytochemistry/ Immunofluorescence: iNOS Antibody (3L0U6) [NBP3-16273]

Immunocytochemistry/Immunofluorescence: iNOS Antibody (3L0U6) [NBP3-16273] - Immunofluorescence analysis of Raw264 7 cells using iNOS Rabbit mAb (NBP3-16273) at dilution of 1:100 (40x lens). Raw264 7 cells were treated by LPS (1 ug/mL) at 37C for 8 hours. Blue: DAPI for nuclear staining.

Applications for iNOS Antibody (3L0U6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: iNOS

Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, nNOS (type I) and endothelial cell NOS, eNOS (type III); along with the inducible isoform, iNOS (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), heme, and tetrahydrobiopterin (1).

The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).

References

1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489

2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174

3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483

Long Name

Inducible Nitic Oxide Synthase

Alternate Names

NOS2, NOS2A

Gene Symbol

NOS2

Additional iNOS Products

Product Documents for iNOS Antibody (3L0U6)

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Product Specific Notices for iNOS Antibody (3L0U6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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