Insect Cathepsin L Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2259

R&D Systems, part of Bio-Techne
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MAB2259
MAB2259-SP
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Key Product Details

Species Reactivity

Validated:

Insect

Cited:

Insect, Insect - Drosophila

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 193720

View all Cathepsin L Antibodies »

Product Specifications

Immunogen

S. frugiperda ovarian cell line Sf 21-derived Insect Cathepsin L

Specificity

Detects pro form of Insect Cathepsin L in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Cathepsin S is observed and no cross-reactivity with rhCathepsin B, F, K, L2/V, and activated, mature insect Cathepsin L is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Insect Cathepsin L Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Insect Cathepsin L, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Insect Cathepsin L
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin L

The pro form of insect cell Cathepsin L is stable at neutral pH, but autoactivates in acidic buffers under reducing conditions. The activated enzyme is similar in substrate specificity to mammalian Cathepsin L, and is very active as a protease, as judged by its ability to degrade azocasein. Cystatins A, B, C, E/M, F, SA, and SN are potent inhibitors of the insect cell Cathepsin L. Like other cysteine proteases related to papain, insect cell Cathepsin L is also inhibited by E-64 and reagents which react with thiols.

References

  1. Johnson, G.D. and W. Jiang (2005) Arch. Biochem. Biophys.444:7.

Alternate Names

CTSL, CTSL1

Entrez Gene IDs

1514 (Human); 13039 (Mouse); 118276442 (Insect)

Gene Symbol

CTSL

Additional Cathepsin L Products

Product Documents for Insect Cathepsin L Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Insect Cathepsin L Antibody

For research use only

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